Genotyping and PCR detection of potential virulence genes in Campylobacter jejuni and Campylobacter coli isolates from different sources in Poland
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- Campylobacter coli klasifikace genetika izolace a purifikace patogenita MeSH
- Campylobacter jejuni klasifikace genetika izolace a purifikace patogenita MeSH
- faktory virulence genetika MeSH
- fylogeneze MeSH
- genotyp MeSH
- kampylobakterové infekce mikrobiologie veterinární MeSH
- kur domácí MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- nemoci drůbeže mikrobiologie MeSH
- nemoci prasat mikrobiologie MeSH
- nemoci psů mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- prasata MeSH
- psi MeSH
- techniky typizace bakterií metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Polsko MeSH
- Názvy látek
- faktory virulence MeSH
The prevalence of potential virulence markers was determined among the population of Polish Campylobacter jejuni and Campylobacter coli isolates from children, chickens, pigs and dogs. The presence of the flaA, flaB, cdtA, cdtB, cdtC, cdtABC, virB11, and cj0588 genes among 74 C. jejuni and 15 C. coli isolates was detected by PCR. High prevalence of five different putative virulence and toxin genes (flaA, cdtA, cdtB, cdtC, and cj0588) was found among isolates obtained from children, chickens and dogs. The occurrence of these genes among isolates obtained from pigs was significantly different than for strains isolated from other sources. Two methods for genotyping Campylobacter spp. strains were applied - flaA-typing, and ADSRRS-fingerprinting method, which was used for the first time for Campylobacter spp. strains. Similarity of the genetic profiles was demonstrated in strains isolated from chickens and dogs, and in isolates from chickens and children. Strains isolated from pigs, both C. jejuni as well as C. coli, did not group with isolates from other sources.
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