Species identification of Campylobacter jejuni ssp. jejuni and C. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and PCR
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články
- MeSH
- Campylobacter coli klasifikace genetika MeSH
- Campylobacter jejuni klasifikace genetika MeSH
- DNA bakterií analýza izolace a purifikace MeSH
- druhová specificita MeSH
- feces mikrobiologie MeSH
- kočky MeSH
- lidé MeSH
- polymerázová řetězová reakce * MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- techniky typizace bakterií * MeSH
- zvířata MeSH
- Check Tag
- kočky MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- DNA bakterií MeSH
The Campylobacter species strains (n = 42; isolated from clinical samples and deposited in Czech National Collection of Type Cultures, Prague) originally phenotypically (and biochemically) identified as Campylobacter jejuni were re-classified using molecular biological and mass spectrometric methods. Whole-cell MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) separated the isolates into two genetically related strains--C. jejuni (n = 26) and C. coli (n = 16) and, moreover, distinguished the intimate details in the group of tested strains. It also made it possible to create the MALDI-TOF MS dendrogram; similarly, the spectral characteristics were used for the 3D cluster analysis. Polymerase chain reaction (PCR) confirmed the results obtained by mass spectrometry. Both methods (PCR and MALDI-TOF MS) gave the same results which supports their suitability in the rapid and accurate Campylobacter-species determination.
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