Susceptibility of Campylobacter jejuni to organic acids and monoacylglycerols
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antibakteriální látky farmakologie MeSH
- barvení a značení metody MeSH
- benzothiazoly MeSH
- buněčná membrána ultrastruktura MeSH
- Campylobacter jejuni účinky léků fyziologie ultrastruktura MeSH
- chinoliny MeSH
- diaminy MeSH
- inhibiční koncentrace 50 MeSH
- kyseliny karboxylové farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- monoglyceridy farmakologie MeSH
- organické látky metabolismus MeSH
- permeabilita buněčné membrány účinky léků MeSH
- počet mikrobiálních kolonií metody MeSH
- polymerázová řetězová reakce MeSH
- transmisní elektronová mikroskopie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- benzothiazoly MeSH
- chinoliny MeSH
- diaminy MeSH
- kyseliny karboxylové MeSH
- monoglyceridy MeSH
- organické látky MeSH
- SYBR Green I MeSH Prohlížeč
Organic acids can be used as feed supplements or for treatment of poultry carcasses in processing plants. The antimicrobial activity of nineteen organic acids and two monoacylglycerols in cultures of Campylobacter jejuni CCM 6214(T) (ATCC 33560) was determined using a SYBR Green-based real-time PCR assay. The IC(50) was a concentration at which only 50 % of a bacteria specific DNA sequence was amplified. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC(50) < or = 0.1 mg/mL). In a weakly acidic environment (pH 5.5), the antimicrobial activity was more pronounced than at pH 6.5. At pH 5.5, oleic and fumaric acid also had clear antimicrobial activity, as did monocaprylin. The antimicrobial activity of acetic, butyric, stearic and succinic acid was low. In cells treated with fumaric acid, the potential of potassium and tetraphenylphosphonium ion-selective electrodes changed, indicating an increase in cytoplasmic and outer membrane permeability, respectively. No changes in membrane permeability were observed in cells treated with capric acid or monocaprin. Transmission electron microscopy revealed separation of the inner and outer membrane in cells treated with capric and fumaric acid, as well as cytoplasmic disorganization in cells exposed to capric acid.
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J Microbiol Methods. 2004 Nov;59(2):173-9 PubMed
J Food Prot. 2000 Sep;63(9):1282-6 PubMed
Int J Food Microbiol. 2007 Apr 20;115(3):268-80 PubMed
Anaerobe. 2009 Feb-Apr;15(1-2):18-25 PubMed
Poult Sci. 2009 Jan;88(1):61-4 PubMed
Foodborne Pathog Dis. 2005 Summer;2(2):160-8 PubMed
Br J Biomed Sci. 1996 Dec;53(4):290-3 PubMed
Avian Pathol. 2008 Aug;37(4):379-83 PubMed
J Bacteriol. 1985 Apr;162(1):42-6 PubMed
Nucl Recept Signal. 2003;1:e012 PubMed
Folia Microbiol (Praha). 2008;53(5):403-9 PubMed
Poult Sci. 2006 Sep;85(9):1570-5 PubMed
Poult Sci. 2002 May;81(5):621-8 PubMed
Meat Sci. 2006 Sep;74(1):89-97 PubMed
Poult Sci. 2003 Apr;82(4):632-9 PubMed
Vet Microbiol. 2004 Apr 19;99(3-4):259-67 PubMed
Symp Ser Soc Appl Microbiol. 2001;(30):96S-114S PubMed
Appl Environ Microbiol. 2000 Sep;66(9):4029-36 PubMed
Folia Microbiol (Praha). 2008;53(3):189-94 PubMed
