Susceptibility of Campylobacter jejuni to organic acids and monoacylglycerols
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Staining and Labeling methods MeSH
- Benzothiazoles MeSH
- Cell Membrane ultrastructure MeSH
- Campylobacter jejuni drug effects physiology ultrastructure MeSH
- Quinolines MeSH
- Diamines MeSH
- Inhibitory Concentration 50 MeSH
- Carboxylic Acids pharmacology MeSH
- Microbial Sensitivity Tests MeSH
- Monoglycerides pharmacology MeSH
- Organic Chemicals metabolism MeSH
- Cell Membrane Permeability drug effects MeSH
- Colony Count, Microbial methods MeSH
- Polymerase Chain Reaction MeSH
- Microscopy, Electron, Transmission MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Benzothiazoles MeSH
- Quinolines MeSH
- Diamines MeSH
- Carboxylic Acids MeSH
- Monoglycerides MeSH
- Organic Chemicals MeSH
- SYBR Green I MeSH Browser
Organic acids can be used as feed supplements or for treatment of poultry carcasses in processing plants. The antimicrobial activity of nineteen organic acids and two monoacylglycerols in cultures of Campylobacter jejuni CCM 6214(T) (ATCC 33560) was determined using a SYBR Green-based real-time PCR assay. The IC(50) was a concentration at which only 50 % of a bacteria specific DNA sequence was amplified. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC(50) < or = 0.1 mg/mL). In a weakly acidic environment (pH 5.5), the antimicrobial activity was more pronounced than at pH 6.5. At pH 5.5, oleic and fumaric acid also had clear antimicrobial activity, as did monocaprylin. The antimicrobial activity of acetic, butyric, stearic and succinic acid was low. In cells treated with fumaric acid, the potential of potassium and tetraphenylphosphonium ion-selective electrodes changed, indicating an increase in cytoplasmic and outer membrane permeability, respectively. No changes in membrane permeability were observed in cells treated with capric acid or monocaprin. Transmission electron microscopy revealed separation of the inner and outer membrane in cells treated with capric and fumaric acid, as well as cytoplasmic disorganization in cells exposed to capric acid.
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