Distinct rpsC single nucleotide polymorphism lineages of Flavescence dorée subgroup 16SrV-D phytoplasma co-infect Vitis vinifera L
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacterial Proteins genetics MeSH
- DNA, Bacterial chemistry genetics MeSH
- DNA Fingerprinting MeSH
- Phylogeny MeSH
- Polymorphism, Single Nucleotide * MeSH
- Molecular Sequence Data MeSH
- Plant Diseases microbiology MeSH
- Phytoplasma classification genetics isolation & purification MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- DNA, Ribosomal genetics MeSH
- Ribosomal Proteins genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology MeSH
- Cluster Analysis MeSH
- Vitis microbiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Italy MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- DNA, Ribosomal MeSH
- Ribosomal Proteins MeSH
- RNA, Ribosomal, 16S MeSH
During a survey on grapevine yellows disease complex in vineyards of Lombardy region (northern Italy), phytoplasmas associated with Flavescence dorée disease were identified in symptomatic grapevines. Polymerase chain reaction and restriction fragment length polymorphism (RFLP) analyses of 16S rDNA revealed the prevalence of phytoplasmal subgroup 16SrV-D. Bioinformatic analyses of nucleotide sequences of rplV and rpsC genes, amplified from 16SrV-D phytoplasma infected grapevines and cloned, underscored the presence of five confirmed rpsC single nucleotide polymorphism (SNP) lineages, determined by different combination of SNPs at nucleotide positions 29, 365, 680, and 720 of rpsC gene. Virtual and actual RFLP analyses with the enzyme TaqI validated the presence of these SNPs. Co-infections by up to four distinct rpsC SNP lineages of 16SrV-D phytoplasma were found in grapevines. These results could open new perspectives for the study of the ecology and the epidemiology of Flavescence dorée.
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GENBANK
EF608223, EF608224, EF608225, EF608227, EF608228
