Development of a SCAR marker by inter-simple sequence repeat for diagnosis of dwarf bunt of wheat and detection of Tilletia controversa KüHN
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- Basidiomycota klasifikace genetika izolace a purifikace MeSH
- DNA fungální chemie genetika MeSH
- DNA primery genetika MeSH
- molekulární sekvence - údaje MeSH
- mykologie metody MeSH
- nemoci rostlin mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- polymorfismus genetický * MeSH
- pšenice mikrobiologie MeSH
- repetitivní sekvence nukleových kyselin MeSH
- sekvence nukleotidů MeSH
- senzitivita a specificita MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA fungální MeSH
- DNA primery MeSH
Dwarf bunt of wheat, caused by Tilletia controversa KüHN: , is a destructive disease on wheat as well as an important internationally quarantined disease in many countries. The primer ISSR818 generated a polymorphic pattern displaying a 867-bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF3/TCKSR3) designed for use in PCR detection assays; they amplified a unique DNA fragment in all isolates of T. controversa but not in the related pathogens. The detection limit with the primer set (TCKSF3/TCKSR3) was 5 ng of DNA which could be obtained from 5.5 microg of teliospores in a 25-microL PCR reaction mixture.
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