Cyclodextrin-based bimodal fluorescence/MRI contrast agents: an efficient approach to cellular imaging
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20583046
DOI
10.1002/chem.200903519
Knihovny.cz E-zdroje
- MeSH
- cyklodextriny chemie MeSH
- fluoresceiny chemie MeSH
- fluorescenční barviva chemie MeSH
- gadolinium chemie MeSH
- kontrastní látky chemie MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- magnetická rezonanční tomografie metody MeSH
- molekulární modely MeSH
- organokovové sloučeniny chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklodextriny MeSH
- fluoresceiny MeSH
- fluorescenční barviva MeSH
- gadolinium MeSH
- kontrastní látky MeSH
- organokovové sloučeniny MeSH
A novel bimodal fluorescence/MRI probe based on a cyclodextrin scaffold has been synthesized and characterized. The final agent employs the fluorescein (F) functionality as a fluorescence marker and the Gd(III) complex of a macrocyclic DOTA-based ligand (GdL) having one aminobenzyl-phosphinic acid pendant arm as an MRI probe, and has a statistical composition of (GdL)(6.9)-F(0.1)-beta-CD. Slow rotational dynamics (governed by a very rigid cyclodextrin scaffold) combined with fast water exchange (ensured by the chosen macrocyclic ligand) resulted in a high relaxivity of approximately 22 s(-1) mM(-1) per Gd(III) or approximately 150 s(-1) mM(-1) per molecule of the final conjugate (20 MHz, 25 degrees C). In vitro labelling of pancreatic islets (PIs) and rat mesenchymal stem cells has been successfully performed. The agent is not cytotoxic and is easily internalized into cells. The labelled cells can be visualized by MRI, as proved by the detection of individual labelled PIs. A fluorescence study performed on mesenchymal stem cells showed that the agent stays in the intracellular space for a long time.
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