PURPOSE: Normal corneal hydration is necessary for the maintenance of corneal transparency. Damage of the corneal epithelium or endothelium by various external influences disturbs the mechanism by which the cornea maintains normal hydration and transparency. The cornea swells, and the corneal thickness increases, resulting in increased scatter and the development of corneal opacity. The transmission of light across the cornea is changed. The purpose of this study is to investigate spectrophotometrically the corneal light transmission under the influence of the various factors affecting the cornea. METHODS: We developed a spectrophotometric method to measure the light transmission across the cornea under the influence of various factors affecting the cornea, such as treatment with 0.9% NaCl, saline, or phosphate buffered saline (PBS), solutions employed as placebo eye drops (negative controls) in experimental studies, agents toxic to the cornea, such as diluted acids or alkalis. The method distinguishes between changes in corneal light transmission caused by altered corneal thickness (the level of hydration) and changes resulting from other corneal disturbances which in turn affect corneal light transmission. RESULTS: The results obtained show that the corneal light transmission is decreased following the application of toxic substances on the corneal surface. This decrease is highly dependent on the severity of the corneal injury evoked by individual noxes, and the resulting changes in corneal hydration and transparency. CONCLUSIONS: The influence of various influences applied to the cornea, manifested as changes in corneal light transmission, can be measured using our spectrophotometric method with a high degree of sensitivity.

Laboratory of Eye Histochemistry and Pharmacology Institute of Experimental Medicine Center of Excellence Academy of Sciences of the Czech Republic Vídeňská 1083 14220 Prague 4 Czech Republic

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Invest Ophthalmol Vis Sci. 2007 Sep;48(9):4061-9 PubMed

Am J Physiol Cell Physiol. 2007 Jul;293(1):C75-86 PubMed

Eye (Lond). 2003 Nov;17(8):927-36 PubMed

Exp Eye Res. 1988 May;46(5):765-8 PubMed

Cutan Ocul Toxicol. 2009;28(3):119-24 PubMed

Biophys J. 2003 Oct;85(4):2205-12 PubMed

Histochemistry. 1989;92(5):441-8 PubMed

Histol Histopathol. 2000 Oct;15(4):1043-50 PubMed

Eur J Ophthalmol. 2001 Apr-Jun;11(2):105-15 PubMed

Acta Ophthalmol (Copenh). 1984 Apr;62(2):309-14 PubMed

Optom Vis Sci. 2006 Jan;83(1):22-6 PubMed

Histol Histopathol. 2005 Apr;20(2):467-73 PubMed

Am J Pathol. 2005 May;166(5):1405-18 PubMed

Curr Eye Res. 1996 Apr;15(4):411-21 PubMed

Br J Ophthalmol. 2007 Nov;91(11):1460-3 PubMed

Toxicology. 2003 Mar 3;184(2-3):141-7 PubMed

Acta Ophthalmol Scand. 1998 Dec;76(6):675-8 PubMed

Chem Biol Interact. 2003 Feb 1;143-144:45-53 PubMed

Ophthalmic Res. 2006;38(2):105-14 PubMed

Biophys J. 1991 Aug;60(2):467-74 PubMed

Photochem Photobiol. 1993 Apr;57(4):663-6 PubMed

Optom Vis Sci. 2000 Jul;77(7):347-56 PubMed

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1976;200(3):251-5 PubMed

Cornea. 1992 Nov;11(6):560-6 PubMed

Cornea. 1998 Nov;17(6):627-39 PubMed

Mol Vis. 2009;15:577-83 PubMed

Biomed Eng Online. 2005 Dec 22;4:70 PubMed

J Opt Soc Am. 1975 May;65(5):524-30 PubMed

Cornea. 2008 Dec;27(10):1182-5 PubMed

Photochem Photobiol. 2009 May-Jun;85(3):794-800 PubMed

Cornea. 2008 May;27(4):504-6 PubMed

Cornea. 2008 Feb;27(2):236-7 PubMed

J Physiol. 1957 Apr 30;136(2):263-86 PubMed

Mol Ther. 2010 Mar;18(3):519-27 PubMed

Int J Pharm. 2002 Jan 31;232(1-2):139-47 PubMed

J Fr Ophtalmol. 2008 Sep;31(7):723-34 PubMed

Clin Exp Ophthalmol. 2008 Oct;36(7):692-4 PubMed

Prog Biophys Mol Biol. 2006 Sep;92(1):80-5 PubMed

Exp Eye Res. 2004 May;78(5):1007-14 PubMed

Ann Ophthalmol. 1979 May;11(5):765-9 PubMed

Am J Optom Physiol Opt. 1984 Jul;61(7):473-8 PubMed

Physiol Res. 2010;59(4):591-597 PubMed

Photochem Photobiol. 1989 Feb;49(2):185-96 PubMed

Photochem Photobiol. 2007 May-Jun;83(3):652-7 PubMed

Exp Eye Res. 1990 Apr;50(4):393-5 PubMed

Cornea. 2009 Jun;28(5):541-6 PubMed

Br J Ophthalmol. 2003 Feb;87(2):225-36 PubMed

Acta Ophthalmol Scand. 2004 Dec;82(6):714-7 PubMed

Cornea. 2005 Aug;24(6):710-6 PubMed

Invest Ophthalmol Vis Sci. 1993 Feb;34(2):349-54 PubMed

Biophys J. 2008 Dec;95(11):5092-9 PubMed

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