Quadruplexes of human telomere dG(3)(TTAG(3))(3) sequences containing guanine abasic sites
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20643102
DOI
10.1016/j.bbrc.2010.07.055
PII: S0006-291X(10)01366-5
Knihovny.cz E-resources
- MeSH
- Circular Dichroism MeSH
- Potassium chemistry MeSH
- G-Quadruplexes * MeSH
- Guanine chemistry MeSH
- Nucleic Acid Conformation * MeSH
- Humans MeSH
- Base Sequence MeSH
- Sodium chemistry MeSH
- Telomere chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Potassium MeSH
- Guanine MeSH
- Sodium MeSH
This study was performed to evaluate how the loss of a guanine base affects the structure and stability of the three-tetrad G-quadruplex of 5'-dG(3)(TTAG(3))(3), the basic quadruplex-forming unit of the human telomere DNA. None of the 12 possible abasic sites hindered the formation of quadruplexes, but all reduced the thermodynamic stability of the parent quadruplex in both NaCl and KCl. The base loss did not change the Na(+)-stabilized intramolecular antiparallel architecture, based on CD spectra, but held up the conformational change induced in dG(3)(TTAG(3))(3) in physiological concentration of KCl. The reduced stability and the inhibited conformational transitions observed here in vitro for the first time may predict that unrepaired abasic sites in G-quadruplexes could lead to changes in the chromosome's terminal protection in vivo.
References provided by Crossref.org
i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions
Loss of loop adenines alters human telomere d[AG3(TTAG3)3] quadruplex folding
