Diversity of anaerobic fungi within cow manure determined by ITS1 analysis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anaerobiosis MeSH
- Biodiversity * MeSH
- DNA, Fungal chemistry genetics MeSH
- DNA Primers genetics MeSH
- RNA, Fungal genetics MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- Manure microbiology MeSH
- Fungi classification genetics isolation & purification physiology MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Molecular Sequence Data MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 5.8S genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Fungal MeSH
- DNA Primers MeSH
- RNA, Fungal MeSH
- Manure MeSH
- DNA, Ribosomal Spacer MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 5.8S MeSH
The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven% of sequences were affiliated with Cyllamyces, 24% with Piromyces, 7% with Anaeromyces, only 2% with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITSbased phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated.
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