Diversity of anaerobic fungi within cow manure determined by ITS1 analysis
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- anaerobióza MeSH
- biodiverzita * MeSH
- DNA fungální chemie genetika MeSH
- DNA primery genetika MeSH
- fungální RNA genetika MeSH
- fylogeneze MeSH
- geny rRNA MeSH
- hnůj mikrobiologie MeSH
- houby klasifikace genetika izolace a purifikace fyziologie MeSH
- mezerníky ribozomální DNA chemie genetika MeSH
- molekulární sekvence - údaje MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 5.8S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA fungální MeSH
- DNA primery MeSH
- fungální RNA MeSH
- hnůj MeSH
- mezerníky ribozomální DNA MeSH
- ribozomální DNA MeSH
- RNA ribozomální 5.8S MeSH
The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven% of sequences were affiliated with Cyllamyces, 24% with Piromyces, 7% with Anaeromyces, only 2% with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITSbased phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated.
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GENBANK
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