Developing biochemical and molecular markers for cyanobacterial inoculants
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence MeSH
- disacharidy metabolismus MeSH
- DNA fingerprinting metody MeSH
- DNA primery MeSH
- fylogeneze MeSH
- mikrobiální testy citlivosti MeSH
- molekulární sekvence - údaje MeSH
- polymerázová řetězová reakce metody MeSH
- půdní mikrobiologie * MeSH
- rhizosféra * MeSH
- sinice klasifikace účinky léků genetika metabolismus MeSH
- zemědělství metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- disacharidy MeSH
- DNA primery MeSH
Markers for evaluating the establishment of cyanobacteria based on their sensitivity or resistance to antibiotics, saccharide utilization patterns and PCR generated fingerprints were developed. Four selected strains (isolates from rhizosphere soils of diverse agro-ecosystems) have shown potential as diazotrophs and exhibited plant growth promoting abilities. Different responses were obtained on screening against 40 antibiotics, which aided in developing selectable antibiotic markers for each strain. Biochemical profiles generated using standardized chromogenic identification system (including saccharide utilization tests) revealed that 53 % of the saccharides tested were not utilized by any strain, while some strains exhibited unique ability for utilization of saccharides such as melibiose, cellobiose, maltose and glucosamine. PCR based amplification profiles developed using a number of primers based on repeat sequences revealed the utility of 3 primers in providing unique fingerprints for the strains.
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J Basic Microbiol. 2008 Jun;48(3):186-94 PubMed
FEMS Microbiol Lett. 1998 Apr 15;161(2):285-92 PubMed
J Biol Chem. 1953 Sep;204(1):197-205 PubMed
Folia Microbiol (Praha). 2004;49(1):26-30 PubMed
Bacteriol Rev. 1971 Jun;35(2):171-205 PubMed
J Bacteriol. 1967 Oct;94(4):972-83 PubMed
J Gen Microbiol. 1965 Apr;39:11-20 PubMed
J Bacteriol. 1995 May;177(9):2387-95 PubMed
Environ Microbiol. 2000 Feb;2(1):11-26 PubMed
Microbiology (Reading). 1998 Oct;144 ( Pt 10):2791-2801 PubMed
Indian J Microbiol. 2008 Mar;48(1):89-94 PubMed
Folia Microbiol (Praha). 2006;51(1):50-6 PubMed
Indian J Microbiol. 2009 Mar;49(1):89-97 PubMed
Folia Microbiol (Praha). 2005;50(5):363-91 PubMed
Arch Mikrobiol. 1972;87(4):303-22 PubMed
J Gen Microbiol. 1967 Nov;49(2):301-13 PubMed
J Basic Microbiol. 2009 Apr;49(2):165-77 PubMed
Microbiol Res. 2006;161(3):187-202 PubMed
Can J Microbiol. 2008 Sep;54(9):701-17 PubMed
J Bacteriol. 1990 May;172(5):2755-61 PubMed
