N-acetyl-D-glucosamine-coated polyamidoamine dendrimer promotes tumor-specific B cell responses via natural killer cell activation
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21349367
DOI
10.1016/j.intimp.2011.02.009
PII: S1567-5769(11)00100-7
Knihovny.cz E-zdroje
- MeSH
- acetylglukosamin chemie farmakologie MeSH
- antigeny CD80 biosyntéza genetika MeSH
- antigeny CD86 biosyntéza genetika MeSH
- B-lymfocyty účinky léků imunologie metabolismus MeSH
- buněčná cytotoxicita závislá na protilátkách účinky léků imunologie MeSH
- buňky NK účinky léků imunologie metabolismus MeSH
- dendrimery chemie farmakologie MeSH
- imunoglobulin G biosyntéza krev genetika imunologie MeSH
- interferon gama biosyntéza genetika MeSH
- interleukin-4 biosyntéza genetika MeSH
- melanom experimentální genetika imunologie metabolismus MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- upregulace účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- acetylglukosamin MeSH
- antigeny CD80 MeSH
- antigeny CD86 MeSH
- Cd86 protein, mouse MeSH Prohlížeč
- dendrimery MeSH
- imunoglobulin G MeSH
- interferon gama MeSH
- interleukin-4 MeSH
- PAMAM Starburst MeSH Prohlížeč
N-acetyl-D-glucosamine-coated polyamidoamine dendrimer (GN8P), exerting high binding affinity to rodent recombinant NKR-P1A and NKR-P1C activating proteins, was shown previously to delay the development of rat colorectal carcinoma as well as mouse B16F10 melanoma, and to potentiate antigen-specific antibody formation in healthy C57BL/6 mice via NK cell stimulation. In this study, we investigated whether GN8P also modulates tumor-specific B cell responses. Serum anti-B16F10 melanoma IgG levels, IgG2a mRNA expression, antibody dependent cell-mediated cytotoxicity (ADCC), and counts of plasma as well as antigen presenting B cells were evaluated in tumor-bearing C57BL/6 mice treated with GN8P and in respective controls. To reveal the mechanism of GN8P effects, the synthesis of interferon-gamma (IFN-γ) and interleukin-4 (IL-4), cytokines involved in regulation of immunoglobulin class switch, was determined. The GN8P treatment significantly elevated IgG, and particularly IgG2a, response against B16F10 melanoma, which led to augmented ADCC reaction. The significant increase in production of IFN-γ, which is known to support IgG2a secretion, was observed solely in NK1.1 expressing cell populations, predominantly in NK cells. Moreover, GN8P raised the number of plasma cells, and promoted antigen presenting capacity of I-A/I-E-positive B lymphocytes by up-regulation of their CD80 and CD86 co-stimulatory molecule expression. These results indicate that GN8P-induced enhancement of tumor-specific antibody formation is triggered by NK cell activation, and contributes to complexity of anticancer immune response involving lectin-saccharide interaction.
Citace poskytuje Crossref.org
Nkrp1 family, from lectins to protein interacting molecules
