T-helper cell type-1 transcription factor T-bet is upregulated in pulmonary sarcoidosis
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21540308
DOI
10.1183/09031936.00089910
PII: 09031936.00089910
Knihovny.cz E-zdroje
- MeSH
- bronchoalveolární lavážní tekutina chemie cytologie MeSH
- chemokin CCL5 genetika metabolismus MeSH
- dospělí MeSH
- exprese genu MeSH
- interferon gama genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické uzliny metabolismus MeSH
- messenger RNA metabolismus MeSH
- plíce metabolismus MeSH
- plicní sarkoidóza genetika imunologie metabolismus MeSH
- proteiny T-boxu metabolismus MeSH
- receptory chemokinů genetika metabolismus MeSH
- receptory CXCR3 genetika metabolismus MeSH
- receptory CXCR6 MeSH
- receptory interleukinu-2 genetika metabolismus MeSH
- Th1 buňky imunologie metabolismus MeSH
- transkripční faktor T-bet MeSH
- upregulace * MeSH
- virové receptory genetika metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- CCL5 protein, human MeSH Prohlížeč
- chemokin CCL5 MeSH
- CXCR6 protein, human MeSH Prohlížeč
- interferon gama MeSH
- messenger RNA MeSH
- proteiny T-boxu MeSH
- receptory chemokinů MeSH
- receptory CXCR3 MeSH
- receptory CXCR6 MeSH
- receptory interleukinu-2 MeSH
- transkripční faktor T-bet MeSH
- virové receptory MeSH
Upregulation of genes for interferon (IFN)-γ and CXC chemokine receptor (CXCR)3 expression, two crucial molecules in sarcoid inflammation and granuloma formation, is directly controlled by the T-helper (Th)1 transcription factor T-bet (T-box, expressed in T-cells). However, there is no information on T-bet expression in sarcoidosis or its relationship with "sarcoidosis-associated" genes. Therefore, we investigated expression of T-bet mRNA and, in parallel, a spectrum of genes known to be involved in sarcoidosis pathogenesis. Transcripts were determined in bronchoalveolar lavage (BAL) cells from 62 sarcoidosis patients and 25 controls by quantitative RT-PCR; T-bet protein was localised by immunohistochemistry. Patient's BAL cells expressed higher mRNA T-bet levels than those of controls (mean ± sd fold change 3.64 ± 1.72; p = 0.00006). T-bet mRNA expression did not vary between clinical phenotypes as assessed by chest radiography stage, presence/absence of Löfgren's syndrome, extrapulmonary/pulmonary involvement or progressing/remitting disease (p > 0.05). T-bet mRNA expression correlated with expression of IFN-γ, CC chemokine ligand 5, CXC chemokine ligand (CXC)10, interleukin (IL)-2 receptor/IL-15 receptor β, CXCR3 and CXCR6 (p < 0.01). T-bet protein was localised to alveolar macrophages and lymphocytes, tissue multinucleated giant cells, macrophages and lymphocytes. In pulmonary sarcoidosis, T-bet upregulation is associated with changes in expression of IFN-γ, CXCR3 and chemokines/receptors involved in the pathogenesis of sarcoidosis, which suggests a role for T-bet in this Th1 disease, including modulation of some sarcoidosis-associated genes.
Eur Respir J. 2012 Jan;39(1):230 PubMed
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