Biotin-modified glutathione as a functionalized coating for bioconjugation of CdTe-based quantum dots
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biotin chemistry metabolism MeSH
- Electrophoresis, Capillary MeSH
- Fluorescence MeSH
- Glutathione chemistry metabolism MeSH
- Quantum Dots * MeSH
- Glutamic Acid chemistry MeSH
- Cadmium Compounds chemistry MeSH
- Streptavidin chemistry MeSH
- Tellurium chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biotin MeSH
- cadmium telluride MeSH Browser
- Glutathione MeSH
- Glutamic Acid MeSH
- Cadmium Compounds MeSH
- sodium tellurate(IV) MeSH Browser
- Streptavidin MeSH
- Tellurium MeSH
In this study, biotin-conjugated glutathione was synthesized using peptide bonding of the biotin carboxy group and amino group of the γ-glutamic acid to prepare an alternative coating for CdTe quantum dots (QDs). This type of coating combines the functionality of the biotin with the fluorescent properties of the QDs to create a specific, high-affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-functionalized glutathione-coated CdTe QDs were prepared by a simple one-step method using Na₂ TeO₃ and CdCl₂. Obtained QDs were separated from the excess of the biotin-conjugated glutathione by CE employing 300 mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and LIF employing Ar⁺ ion laser (488 nm).
References provided by Crossref.org
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