Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arabidopsis enzymology genetics MeSH
- Cell Division MeSH
- Down-Regulation MeSH
- Gene Duplication genetics MeSH
- Phenotype MeSH
- Aurora Kinases MeSH
- Meristem enzymology genetics growth & development MeSH
- Microtubules metabolism MeSH
- Protein Serine-Threonine Kinases metabolism MeSH
- Microtubule-Associated Proteins metabolism MeSH
- Arabidopsis Proteins metabolism MeSH
- RNA Interference MeSH
- Protein Transport MeSH
- Tubulin metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- AUR1 protein, Arabidopsis MeSH Browser
- Aurora Kinases MeSH
- Protein Serine-Threonine Kinases MeSH
- Microtubule-Associated Proteins MeSH
- Arabidopsis Proteins MeSH
- TPX2 protein, Arabidopsis MeSH Browser
- Tubulin MeSH
• The conserved family of Aurora kinases has multiple functions during mitosis. The roles of plant Aurora kinases have been characterized using inhibitor treatments. • We down-regulated Aurora kinases in Arabidopsis thaliana using RNA interference (RNAi). We carried out a detailed phenotypic analysis of Aurora RNAi plants, biochemical and microscopic studies of AtAurora1 kinase together with AtTPX2 (targeting protein for Xklp2) and γ-tubulin. • Cell division defects were observed in plants with reduced expression of Aurora kinases. Furthermore, the maintenance of primary meristems was compromised and RNAi seedlings entered endoreduplication prematurely. AtAurora1, its activator AtTPX2, and γ-tubulin were associated with microtubules in vitro; they were attached to regrowing kinetochore microtubules and colocalized on spindle microtubules and with a subset of early phragmoplast microtubules. Only the AtAurora1 kinase was translocated to the area of the cell plate. • RNAi silencing of Aurora kinases showed that, in addition to their function in regulating mitosis, the kinases are required for maintaining meristematic activity and controlling the switch from meristematic cell proliferation to differentiation and endoreduplication. The colocalization and co-fractionation of AtAurora1 with AtTPX2, and γ-tubulin on microtubules in a cell cycle-specific manner suggests that AtAurora1 kinase may function to phosphorylate substrates that are critical to the spatiotemporal regulation of acentrosomal microtubule formation and organization.
Institute of Experimental Botany AS CR v v i Sokolovská 6 772 00 Olomouc Czech Republic
Institute of Microbiology AS CR v v i Vídeňská 1083 142 20 Prague 4 Czech Republic
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