Differentiation of petroleum hydrocarbon-degrading Pseudomonas spp. based on PCR-RFLP of the 16S-23S rDNA intergenic spacer region
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article
- MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny MeSH
- DNA, Ribosomal Spacer genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Polymorphism, Restriction Fragment Length * MeSH
- Pseudomonas classification genetics isolation & purification metabolism MeSH
- RNA, Ribosomal, 16S genetics MeSH
- RNA, Ribosomal, 23S genetics MeSH
- Petroleum microbiology MeSH
- Bacterial Typing Techniques methods MeSH
- Hydrocarbons metabolism MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
- DNA, Ribosomal Spacer MeSH
- RNA, Ribosomal, 16S MeSH
- RNA, Ribosomal, 23S MeSH
- Petroleum MeSH
- Hydrocarbons MeSH
Genetic diversity among 43 petroleum hydrocarbon-degrading Pseudomonas belonging to four different species and the type strain Pseudomonas aeruginosa MTCC1034 was assessed by using restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified 16S-23S rDNA intergenic spacer regions (ISRs) polymorphism. PCR amplification from all Pseudomonas species yielded almost identical ISR amplicons of "?" 800 bp and in nested PCR of "?" 550 bp. The RFLP analysis with MboI and AluI revealed considerable intraspecific variations within the Pseudomonas species. The dendrogram constructed on the basis of the PCR-RFLP patterns of 16S-23S rDNA intergenic spacer regions differentiated all the species into seven different clusters.
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