Production of giant mouse oocyte nucleoli and assessment of their protein content
Language English Country Japan Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22293324
DOI
10.1262/jrd.2011-004
PII: JST.JSTAGE/jrd/2011-004
Knihovny.cz E-resources
- MeSH
- Cell Nucleolus metabolism physiology MeSH
- Cell Nucleus MeSH
- Cell Culture Techniques MeSH
- Chromatin metabolism MeSH
- Gold Colloid pharmacology MeSH
- Embryo Culture Techniques MeSH
- Mice MeSH
- Oocytes cytology MeSH
- DNA Damage MeSH
- Gene Expression Regulation * MeSH
- Nuclear Transfer Techniques * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- Gold Colloid MeSH
Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses, a sufficient number of oocyte nucleoli is very difficult to obtain. We have found that isolated oocytes nucleoli fuse very efficiently when contact is established between them. Thus, well visible giant nucleoli can be obtained, relatively easily handled and then used for further biochemical analyses. With the use of colloidal gold staining, we estimated that a single fully grown mouse oocyte nucleolus contains approximately 1.6 ng of protein. We do believe that this approach will accelerate further research aiming at analyzing the composition of oocyte nucleoli in more detail.
References provided by Crossref.org
Improving the Quality of Oocytes with the Help of Nucleolotransfer Therapy
Nucleoli in embryos: a central structural platform for embryonic chromatin remodeling?
