The aim of the presented study was to assess the effect of a single administration of Fe(3+)-dextran on immune cell counts and pterin biomolecule production as novel sensors of the piglets' immune system activation, and to determine concentrations of cortisol, a traditional hormonal biosensor of the stress response. Pterins (neopterin and biopterin) in the piglets' blood serum were analyzed by separation using reversed-phase HPLC. A single dose of Fe(3+)-dextran produced a special stress situation in the piglets' organism which manifested itself by an increased production of neopterin (p < 0.05) and biopterin (p < 0.01) in the experimental piglets. Changes in cortisol concentrations and leukocyte counts were influenced by handling stress and were not specifically correlated to iron dextran application. Iron concentrations in the internal environment of the experimental piglets' group were higher by an order of magnitude compared with the controls, and the highest serum concentrations of iron (p < 0.01) were reached 24 h following Fe(3+)-dextran administration. The data presented offer a new perspective on the evaluation of stress situations in the animal organism and, not least importantly, extends the rather modest current list of references on the role of pterins in livestock animals.

Department of Biochemistry Chemistry and Biophysics Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Palackeho 1 3 Brno 612 42 Czech Republic

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Berdovska A., Zwirska-Korczala K. Neopterin measurement in clinical diagnosis. J. Clin. Pharm. Ther. 2001;26:319–329. PubMed

Brown G.M. The biosynthesis of pteridines. Adv. Enzymol. 1971;35:5–77. PubMed

Zimmerman A.W., Jyonouchi H., Comi A.M., Connors S.L., Milstien S., Varsou A. Cerebrospinal fluid and serum markers of inflammation in autism. Pediatr. Neurol. 2005;33:195–201. PubMed

Ziegler I., Fink M., Wilmanns W. Biopterin level in peripheral blood cells as marker for hemopoietic cell proliferation during leukemia and polycythemia vera. Blut. 1982;44:231–240. PubMed

Hashimoto R., Nagatsu T., Ohta T., Mizutani M., Omura I. Changes in the concentrations of tetrahydrobiopterin, the cofactor of tyrosine hydroxylase, in blood under physical stress and depression. Ann. N.Y. Acad. Sci. 2004;1018:378–386. PubMed

Murr C., Fuith L.C., Winder B., Wirleitner B., Baier-Bitterlich G., Fuchs D. Increased neopterin concentrations in patients with cancer: indicator of oxidative stress? Anticancer Res. 1999;19:1721–1728. PubMed

Forrest C.M., Youd P., Kennedy A., Gould S.R., Darlington L.G., Stone T.W. Purine, kynurenine, neopterin and lipid peroxidation levels in inflammatory bowel disease. J. Biomed. Sci. 2002;9:436–442. PubMed

Murr C., Winder B., Wirleitner B., Fuchs D. Neopterin as a marker for immune system activation. Curr. Drug Metab. 2002;3:175–187. PubMed

Fuchs D., Wachter H. International Clinical Laboratory Diagnostics. 1st ed. TH Books Verlagsgesellschaft mbH; Frankfurt, Germany: 1998. Neopterin.

Jerin A., Pozar-Lukanovic N., Sojar V., Stanisavljevic D., Pavern-Erzen V., Osredkar J. Neopterin: an early marker of surgical stress and hypoxic reperfusion damage during liver surgery. Clin. Chem. Lab. Med. 2002;40:663–666. PubMed

Svoboda M. Metabolické poruchy u prasat (Metabolic disorders in pigs) Farmář. 2004;12:35–36.

Guo D., Jaber B.L., Lee S., Perianaygam M.C., King A.J., Periba B.J.G., Balakrishnan V.S. Impact of iron dextran on polymorphonuclear cell function among hemodialysis patients. Clin. Nephrol. 2002;58:134–142. PubMed

Brohee D., Vanhaeverbeek M., Kennes B., Neve P. Leukocyte and lymphocyte subsets after a short pharmacological stress by intravenous epinephrine and hydrocortisone in healthy humans. Int. J. Neurosci. 1990;53:53–62. PubMed

Brock J.H. Iron and immunity. J. Nutr. Immunol. 1993;2:47–106.

Toman M. ‘Veterinární Imunologie (Veterinary Immunology) 1st ed. Grada Publishing; Prague, Czech Republic: 2000. p. 350.

Walker E.M., Jr., Walker S.M. Effects of iron overload on the immune system. Ann. Clin. Lab. Sci. 2000;30:354–365. PubMed

Cardier J.E., Romano E., Soyano A. T lymphocytes subsets in experimental iron overload. Immunopharmacol. Immunotoxicol. 1997;19:75–87. PubMed

Spear A.T., Sherman A.B. Iron deficiency alters DMBA-induced tumor burden and natural killer cell cytotoxicity rats. J. Nutr. 1992;122:46–55. PubMed

Kuvibidila S.R., Kitchens D., Baliga B.S. In vivo and in vitro iron deficiency reduces protein kinase C activity and translocation in murine splenic and purified T cells. J. Cell. Biochem. 1999;74:468–478. PubMed

Werner-Felmayer G., Golderer G., Werner E.R. Tetrahydrobiopterin biosynthesis, utilization and pharmacological effects. Curr. Drug Metab. 2002;3:159–173. PubMed

Razumovitch J.A., Semenkova G.N., Fuchs D., Cherenkevich S.N. Influence of neopterin on the generation of reactive oxygen species in human neutrophils. FEBS Lett. 2003;549:83–86. PubMed

Baier-Bitterlich G., Fuchs D., Murr C.H., Reibnegger G., Werner-Felmayer G., Sgonc R., Bőck G., Dierich M.P., Wachter H. Effect of neopterin and 7,8-dihydroneopterin on tumor necrosis factor-α induced programmed cell death. FEBS Lett. 1995;364:234–238. PubMed

Oettl K., Wirleitner B., Baier-Bitterlich G., Grammer T., Fuchs D., Reibnegger G. Formation of oxygen radicals in solutions of 7,8-dihydroneopterin. Biochem. Biophys. Res. Commun. 1999;264:262–267. PubMed

Schrodl W., Kunze R., Kruger M. Determination of C-reactive protein and neopterin in serum diseased and bacterially infected swine. Berl. Műnch. Tierärzth. Wschr. 1998;111:321–325. PubMed

Amann A., Winder B., Rieder J., Antretter H., Hoffmann G., Mayr V., Strohmenger H.U., Fuchs D. Monitoring of immune activation using biochemical changes in a porcine model of cardiac arrest. Mediat. Inflamm. 2001;10:343–346. PubMed PMC

Fujioka H., Shintaku H., Nakanishi H., Kim T.J., Kusuda S., Yamano T. Biopterin in the acute phase of hypoxia-ischemia in a neonatal pig model. Brain Develop. 2008;30:1–6. PubMed

Carru C., Zinellu A., Sotgia S., Serra R., Usai M.F., Pintus G.F., Pes G.M., Deiana L. A new HPLC methods for serum neopterin measurement and relationship with plasma thiols in healthy subjects. Biomed. Chromatogr. 2004;18:360–366. PubMed