The first application of boromycin as a chiral selector in capillary electrophoresis is described. Given boromycin's insolubility in water, a non-aqueous background electrolyte based on methanol was used for enantiomeric discrimination of selected chiral primary amines (α-methylbenzylamine, R,S-tryptophanol, R,S-norepinephrine, R,S-octopamine, R,S-p-hydroxynorephedrine and R,S-2-amino-1-phenylethanol). A basic study of experimental conditions including the influence of boromycin concentration, the composition and concentration of background electrolyte and also the influence of different organic solvents was performed. The best separation condition was 75 mM Tris/50mM boric acid in methanol, pHws 9.0, with a positive separation voltage. The enantiomeric separation of the primary amines was achieved within 14 min with resolution values greater than 1.5 for the majority of the studied analytes.

Regional Centre of Advanced Materials and Technologies Department of Analytical Chemistry Faculty of Science Palacký University in Olomouc 17 Listopadu 12 Olomouc CZ 77146 Czech Republic

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Macrocyclic glycopeptide-based chiral selectors for enantioseparation in sub/supercritical fluid chromatography