Study on the use of boromycin as a chiral selector in capillary electrophoresis
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22475183
DOI
10.1016/j.chroma.2012.02.073
PII: S0021-9673(12)00380-9
Knihovny.cz E-zdroje
- MeSH
- boritany chemie MeSH
- elektroforéza kapilární metody MeSH
- stereoizomerie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- boritany MeSH
- boromycin MeSH Prohlížeč
The first application of boromycin as a chiral selector in capillary electrophoresis is described. Given boromycin's insolubility in water, a non-aqueous background electrolyte based on methanol was used for enantiomeric discrimination of selected chiral primary amines (α-methylbenzylamine, R,S-tryptophanol, R,S-norepinephrine, R,S-octopamine, R,S-p-hydroxynorephedrine and R,S-2-amino-1-phenylethanol). A basic study of experimental conditions including the influence of boromycin concentration, the composition and concentration of background electrolyte and also the influence of different organic solvents was performed. The best separation condition was 75 mM Tris/50mM boric acid in methanol, pHws 9.0, with a positive separation voltage. The enantiomeric separation of the primary amines was achieved within 14 min with resolution values greater than 1.5 for the majority of the studied analytes.
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