Study on the use of boromycin as a chiral selector in capillary electrophoresis

. 2012 May 11 ; 1237 () : 128-32. [epub] 20120306

Jazyk angličtina Země Nizozemsko Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid22475183
Odkazy

PubMed 22475183
DOI 10.1016/j.chroma.2012.02.073
PII: S0021-9673(12)00380-9
Knihovny.cz E-zdroje

The first application of boromycin as a chiral selector in capillary electrophoresis is described. Given boromycin's insolubility in water, a non-aqueous background electrolyte based on methanol was used for enantiomeric discrimination of selected chiral primary amines (α-methylbenzylamine, R,S-tryptophanol, R,S-norepinephrine, R,S-octopamine, R,S-p-hydroxynorephedrine and R,S-2-amino-1-phenylethanol). A basic study of experimental conditions including the influence of boromycin concentration, the composition and concentration of background electrolyte and also the influence of different organic solvents was performed. The best separation condition was 75 mM Tris/50mM boric acid in methanol, pHws 9.0, with a positive separation voltage. The enantiomeric separation of the primary amines was achieved within 14 min with resolution values greater than 1.5 for the majority of the studied analytes.

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