Inter-species differences in the growth of bifidobacteria cultured on human milk oligosaccharides
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bifidobacterium growth & development isolation & purification metabolism MeSH
- Feces microbiology MeSH
- Infant MeSH
- Culture Media metabolism MeSH
- Humans MeSH
- Milk, Human chemistry microbiology MeSH
- Oligosaccharides metabolism MeSH
- Check Tag
- Infant MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Culture Media MeSH
- Oligosaccharides MeSH
Human milk (HM) contains as the third most abundant component around 200 different structures of human milk oligosaccharides (HMOs). HMOs are the first and irreplaceable prebiotics for infants, supporting bifidobacteria as the most important bacterial group in an infant intestine. The aim of our study was to test the growth of bifidobacteria in HM and on HMOs. Bifidobacteria were isolated from two groups of infants. The first one (eight strains) were isolated from infants who had bifidobacteria in their feces but, after a short period of time (4 to 24 days), bifidobacteria were no longer detected in their feces (disappeared bifidobacteria [DB]). The second group of bifidobacteria (eight strains) originated from infants with continual presence of bifidobacteria in their feces (persistent bifidobacteria [PB]). There were significant differences (p < 0.05) between DB and PB groups in the ability of the strains to grow in HM. PB grew in HM, reaching counts higher than 7 log CFU/ml. In contrast, counts of DB decreased from 5 to 4.3 log CFU/ml after cultivation in HM. The final pH after cultivation of bifidobacteria on HMOs was 6.2 and 4.9 in DP and PB groups, respectively. In general, Bifidobacterium bifidum and B. breve species were able to utilize HMOs, while B. adolescentis and B. longum subsp. longum species did not. The ability to grow in HM and to utilize HMOs seem to be important properties of bifidobacteria which are able to colonize infant intestinal tract.
See more in PubMed
J Nutr. 2005 Jan;135(1):1-4 PubMed
J Perinatol. 2011 Sep;31(9):593-8 PubMed
J Clin Gastroenterol. 2004 Jul;38(6 Suppl):S80-3 PubMed
Am J Clin Nutr. 2003 Jun;77(6):1537S-1543S PubMed
J Microbiol Methods. 2000 Dec 15;43(2):127-32 PubMed
J Microbiol Methods. 2005 Mar;60(3):365-73 PubMed
J Pediatr Gastroenterol Nutr. 2010 Jul;51(1):77-84 PubMed
Biotechnol Lett. 2010 Mar;32(3):451-5 PubMed
Br J Nutr. 2005 Apr;93 Suppl 1:S31-4 PubMed
Multidisciplinary involvement and potential of thermophiles
Mucin Cross-Feeding of Infant Bifidobacteria and Eubacterium hallii
