Resistance in Escherichia coli isolates colonizing gastrointestinal tracts of dogs, cats, and their owners in Northern Kenya was investigated with an emphasis on extended-spectrum beta-lactamases (ESBLs). Totals of 47 (22%, n = 216), 2 (4%, n = 50), and 4 (17%, n = 23) CTX-M-15-producing E. coli isolates were obtained from dogs, cats, and humans, respectively. CTX-M-15-producing E. coli isolates with identical PFGE profiles were detected in animals and humans living in the same area.

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Zobrazit více v PubMed

Barton BM, Harding GP, Zuccarelli AJ. 1995. A general method for detecting and sizing large plasmids. Anal. Biochem. 226:235–240 PubMed

Bett B, Jost Cand Mariner J. 2008. Participatory investigation of important animal health problems amongst the Turkana pastoralists: relative incidence, impact on livelihoods and suggested interventions. Discussion Paper No. 15. Targeting and Innovation. International Livestock Research Institute, Nairobi, Kenya

Caratolli A, et al. 2005. Identification of plasmids by PCR-based replicon typing. J. Microbiol. Methods 63:219–228 PubMed

CDC 2004. Standardized molecular subtyping of foodborne bacterial pathogens by pulse-field gel electrophoresis. Centers for Disease Control, Atlanta, GA

Cleaveland S, Meslin FX, Breiman R. 2006. Dogs can play useful role as sentinel hosts for disease. Nature 440:605. PubMed

Clermont O, Bonacorsi S, Bingen E. 2000. Rapid and simple determination of Escherichia coli phylogenetic group. Appl. Environ. Microbiol. 66:4555–4558 PubMed PMC

Clermont O, et al. 2009. Rapid detection of the O25-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains. J. Antimicrob. Chemother. 64:274–277 PubMed

CLSI 2008. Performance standards for antimicrobial susceptibility testing, 18th informational supplement. CLSI document M100-S18. CLSI, Wayne, PA

Coque TM, et al. 2008. Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15. Emerg. Infect. Dis. 14:195–200 PubMed PMC

Dolejska M., et al. CTX-M-15-producing Escherichia coli clone B2-O25b-ST131 and Klebsiella spp. in municipal wastewater treatment plant effluents. J. Antimicrob. Chemother. 66:2784–2790 PubMed

Eckert C, Gautier V, Arlet G. 2006. DNA sequence analysis of the genetic environment of various blaCTX-M genes. J. Antimicrob. Chemother. 57:14–23 PubMed

Guardabassi L, Schwarz S, Lloyd DH. 2004. Pet animals as reservoirs of antimicrobial-resistant bacteria. J. Antimicrob. Chemother. 54:321–332 PubMed

Hernandez J, et al. 2010. Globally disseminated human pathogenic Escherichia coli of O25-ST131 clone, harboring blaCTX-M-15, found in glaucous-winged gull at remote Commander Islands, Russia. Environ. Microbiol. Rep. 2:329–332 PubMed

Literak I, et al. 2010. Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant Escherichia coli producing extended-spectrum beta-lactamases in wild boars. J. Appl. Microbiol. 108:1702–1711 PubMed

Olesen I, Hasman H, Aarestrup FM. 2004. Prevalence of beta-lactamases among ampicillin-resistant Escherichia coli and Salmonella isolated from food animals in Denmark. Microb. Drug Resist. 10:334–340 PubMed

Pallecchi L, et al. Rapid dissemination and diversity of CTX-M extended-spectrum beta-lactamase genes in commensal Escherichia coli isolates from healthy children from low-resource setting in Latin America. Antimicrob. Agents Chemother. 51:2720–2725 PubMed PMC

Wirth T, et al. 2006. Sex and virulence in Escherichia coli: an evolutionary perspective. Mol. Microbiol. 60:1136–1151 PubMed PMC

Extensive Genetic Commonality among Wildlife, Wastewater, Community, and Nosocomial Isolates of Escherichia coli Sequence Type 131 (H30R1 and H30Rx Subclones) That Carry blaCTX-M-27 or blaCTX-M-15

Low rates of antimicrobial-resistant Enterobacteriaceae in wildlife in Taï National Park, Côte d'Ivoire, surrounded by villages with high prevalence of multiresistant ESBL-producing Escherichia coli in people and domestic animals