The ability of the rumen ciliates to utilize β-glucans other than cellulose and xylan is currently being recognized. The objective of the present study was to characterize the ability of the ciliate Diploplastron affine to digest some pachyman, laminarin, pustulan, curdlan and lichean. The protozoa were isolated from the rumen of sheep and either grown in vitro or inoculated into the rumen of ciliate-free sheep and maintained in natural conditions. In vitro culture studies showed that the enrichment of culture medium with the examined saccharides results in an increase in the number of ciliates in comparison to the control cultures. The increase was over 36 and 15 % when the growth medium was supplemented with pachyman (1,3-β-glucan) and pustulan (1,6-β-glucan), respectively. A positive correlation was also found between the population density of ciliates and the dose of saccharide supplemented to the growth medium. Enzyme studies were performed using the crude enzyme preparation obtained from ciliates treated with antibiotics. The ability of ciliates to digest the examined β-glucans was tested by the quantification of reducing sugars released from the mentioned substrates during the incubation with crude enzyme preparation. The results showed that D. affine ciliates were able to digest both of them. The mean degradation rate varied between 6.7 and 28.2 μmol/L glucose per mg protein per h for pustulan and lichean, respectively, whereas the digestion velocity was the highest at 5.0-5.5 pH and 45-50°C.

The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences 05 110 Jabłonna Poland

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Bailey RW, Gaillard BDE. Carbohydrates of the rumen ciliate Epidinium ecaudatum (Crawley) Biochem J. 1965;95:758–767. PubMed PMC

Bartnicki-Garcia S. Cell wall composition and other biochemical markers in fungal phylogeny. In: Harborne JB, editor. Phytochemical phylogeny. New York: Academic; 1970. pp. 81–103.

Belzecki G, Miltko R, Kwiatkowska E, Michalowski T. Mureinolytic ability of the rumen ciliate Diploplastron affine. Folia Microbiol. 2010;55:312–314. doi: 10.1007/s12223-010-0047-0. PubMed DOI

Beran K, Holan Z, Baldrik J. Chitin-glucan complex in yeast Saccharomyces cerevislae. I. IR and X-ray analysis. Folia Microbiol. 1972;17:322–330. doi: 10.1007/BF02884098. PubMed DOI

Burton RA, Fincher GB. (1,3;1,4)-β-d-Glucans in cell walls of the Poaceae, lower plants, and fungi: a tale of two linkages. Mol Plant. 2009;2:873–882. doi: 10.1093/mp/ssp063. PubMed DOI

Carbonero ER, Smiderle FR, Gracher AHP, Mellinger CG, Torri G, Ahti T, Gorin PAJ, Acomini MI. Structure of two glucans and a galactofuranomannan from the lichen Umbilicaria mammulata. Carbohydr Polym. 2006;63:13–18. doi: 10.1016/j.carbpol.2005.04.010. DOI

Coleman GS, Davies JI, Cash MA. The cultivation of rumen ciliates Epidinium ecaudatum caudatum and Polyplastron multivesiculatum in vitro. J Gen Microbiol. 1972;73:509–521. doi: 10.1099/00221287-73-3-509. PubMed DOI

Dogiel VA. Monographie der Familie Ophryoscolecidae. Arch Protistenk. 1927;59:1–288.

Fincher GB, Stone BA. Cell walls and their components in cereal grain technology. Adv Cereal Sci Technol. 1986;8:207–295.

Martin K, McDougall BM, McIlroy S, Jayus CJ, Seviour RJ. Biochemistry and molecular biology of exocellular fungal β-(1, 3)- and β-(1, 6)-glucanases. FEMS Microbiol Rev. 2007;31:168–192. doi: 10.1111/j.1574-6976.2006.00055.x. PubMed DOI

Michalowski T. A simple system for continuous culture of rumen ciliates. Bull Acad Pol Sci Ser Biol. 1979;27:581–583. PubMed

Michalowski T, Szczepkowski P, Muszynski P. The nutritive factors affecting the growth of the rumen ciliate Diploplastron affine in vitro. Acta Protozool. 1986;25:419–426.

Michalowski T, Harmeyer J, Belzecki G. The importance of washing the omasum for successful defaunation of sheep. J Anim Feed Sci. 1999;8:611–619.

Miller GL, Blum R, Glennon WE, Butron AL. Measurement of carboxymethylcellulase activity. Anal Biochem. 1960;2:127–132. doi: 10.1016/0003-2697(60)90004-X. DOI

Morgavi DP, Sakurada M, Tomita Y, Onodera R. Presence in rumen bacteria and protozoal populations of enzymes capable of degrading fungal cell walls. Microbiology. 1994;140:631–636. doi: 10.1099/00221287-140-3-631. PubMed DOI

Nieminen M, Heiskari U. Diet of freely grazing and captive reindeer during summer and winter. Rangifer. 1989;9:17–34.

Ruszczyc Z. Methods in Zootechnological Research. Warszawa: PWRiL; 1970.

Seichertová O, Beran K, Holan Z, Pokorny V. The chitin-glucan complex of Saccharomyces cerevisiae. II. Location of the complex in the encircling region of the bud scar. Folia Microbiol. 1973;18:207–211. doi: 10.1007/BF02872858. PubMed DOI