Expression patterns of Ruminococcus flavefaciens 007S cellulases as revealed by zymogram approach
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- Bacterial Proteins chemistry genetics metabolism MeSH
- Cellobiose metabolism MeSH
- Cellulases chemistry genetics metabolism MeSH
- Cellulose metabolism MeSH
- Enzyme Assays MeSH
- Gene Expression MeSH
- Molecular Sequence Data MeSH
- Molecular Weight MeSH
- Ruminococcus chemistry enzymology genetics growth & development MeSH
- Protein Structure, Tertiary MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Cellobiose MeSH
- Cellulases MeSH
- Cellulose MeSH
The expression of Ruminococcus flavefaciens 007S cellulases in different incubation time points (growth stages) and their substrate inducibility were analyzed by comparing the zymogram expression profiles of cultures grown on insoluble cellulose (Avicel) with cellobiose-grown cultures. The molecular weights of the enzymes were compared to (putative) cellulases encoded in the R. flavefaciens FD-1 genome.
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