Fluorescence emission spectra of calcofluor stained yeast cell suspensions: heuristic assessment of basis spectra for their linear unmixing
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- barvení a značení MeSH
- benzensulfonáty metabolismus MeSH
- buněčná stěna chemie metabolismus MeSH
- fluorescenční barviva metabolismus MeSH
- fluorescenční spektrometrie MeSH
- glukosa farmakologie MeSH
- Saccharomyces cerevisiae chemie cytologie účinky léků metabolismus MeSH
- suspenze MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- benzensulfonáty MeSH
- C.I. Fluorescent Brightening Agent 28 MeSH Prohlížeč
- fluorescenční barviva MeSH
- glukosa MeSH
- suspenze MeSH
Fluorescence emission spectra of yeast cell suspensions stained with calcofluor have recently been identified as promising markers of variations in the quality of yeast cell wall. It is shown in this paper how the raw fluorescence spectra of calcofluor can be transformed to reliable spectral signatures of cell wall quality, which are independent of actual dye-to-cell concentrations of examined cell suspensions. Moreover, the presented approach makes it possible to assess basis fluorescence spectra that allows for the spectral unmixing of raw fluorescence spectra in terms of respective fluorescence contributions of calcofluor solvated in the suspension medium and bound to yeast cell walls.
Zobrazit více v PubMed
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