Solvatochromic effect in the optical spectra of calcofluor and its relation to fluorescent staining of yeast cell walls
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- absorpce MeSH
- barvení a značení MeSH
- benzensulfonáty chemie metabolismus MeSH
- buněčná stěna metabolismus MeSH
- chemické jevy MeSH
- chitin metabolismus MeSH
- fluorescenční barviva chemie metabolismus MeSH
- fluorescenční spektrometrie MeSH
- koncentrace vodíkových iontů MeSH
- roztoky MeSH
- Saccharomyces cerevisiae cytologie MeSH
- voda chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- benzensulfonáty MeSH
- C.I. Fluorescent Brightening Agent 28 MeSH Prohlížeč
- chitin MeSH
- fluorescenční barviva MeSH
- roztoky MeSH
- voda MeSH
Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
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