Solvatochromic effect in the optical spectra of calcofluor and its relation to fluorescent staining of yeast cell walls
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Absorption MeSH
- Staining and Labeling MeSH
- Benzenesulfonates chemistry metabolism MeSH
- Cell Wall metabolism MeSH
- Chemical Phenomena MeSH
- Chitin metabolism MeSH
- Fluorescent Dyes chemistry metabolism MeSH
- Spectrometry, Fluorescence MeSH
- Hydrogen-Ion Concentration MeSH
- Solutions MeSH
- Saccharomyces cerevisiae cytology MeSH
- Water chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Benzenesulfonates MeSH
- C.I. Fluorescent Brightening Agent 28 MeSH Browser
- Chitin MeSH
- Fluorescent Dyes MeSH
- Solutions MeSH
- Water MeSH
Fluorescence spectral properties of calcofluor (a popular stain used to visualize cell walls of bacteria, yeast and fungi) has been studied. The analysis of calcofluor fluorescence emission spectra measured in a wide range of solvents (including media containing chitin), and in yeast cell suspensions has revealed that the solvatochromic properties of calcofluor ensue essentially from the by solvent-solute hydrogen bonding, or from the hydrogen bonding to cell wall polysaccharides with an eventual contribution of calcofluor aggregation at the cell surface. Preliminary data suggest that calcofluor emission spectra can be employed as a practical marker of variations in the quality of yeast cell wall.
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