Reproducible discrimination between gram-positive and gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22577658
DOI
10.1039/c2an16310a
Knihovny.cz E-resources
- MeSH
- Cell Death MeSH
- Time Factors MeSH
- Gram-Negative Bacteria chemistry cytology isolation & purification MeSH
- Gram-Positive Bacteria chemistry cytology isolation & purification MeSH
- Surface Properties MeSH
- Spectrum Analysis, Raman * MeSH
- Reproducibility of Results MeSH
- Cluster Analysis MeSH
- Spectrophotometry, Infrared MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent re-crystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates.
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