Major clonal lineages in impetigo Staphylococcus aureus strains isolated in Czech and Slovak maternity hospitals
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22664376
DOI
10.1016/j.ijmm.2012.04.001
PII: S1438-4221(12)00017-3
Knihovny.cz E-resources
- MeSH
- Genes, Bacterial * MeSH
- Phenotype MeSH
- Genotype MeSH
- Impetigo microbiology MeSH
- Humans MeSH
- Multilocus Sequence Typing MeSH
- Infant, Newborn MeSH
- Polymorphism, Genetic MeSH
- Hospitals, Maternity * MeSH
- Carrier State microbiology MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Base Sequence MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus aureus classification genetics isolation & purification MeSH
- Bacterial Typing Techniques methods MeSH
- Equipment and Supplies, Hospital microbiology MeSH
- Check Tag
- Humans MeSH
- Infant, Newborn MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czechoslovakia MeSH
One hundred and twenty-seven exfoliative toxin-producing (ET-positive) strains of Staphylococcus aureus collected in 23 Czech and one Slovak maternity hospitals from 1998 to 2011 were genotypically characterized by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) profiling, spa gene polymorphism analysis, and ETA-converting prophage carriage, which resulted in the identification of 21 genotypes grouped into 4 clonal complexes (CC). Ninety-one isolates carried the eta gene alone whilst 12 isolates harboured only the etb gene. Two new, to date not defined, spa types (t6644 and t6645) and 2 novel sequence types (ST2194 and ST2195) were identified in the set of strains under study. The predominant CC121 occurred in 13 Czech hospitals. CC15, CC9, and ST88 (CC88) exclusively included eta gene-positive strains while the strains belonging to ST121 harboured the eta and/or etb genes. This study highlights not only significant genomic diversity among impetigo strains and the distribution of major genotypes disseminated in the Czech and Slovak maternity hospitals, but also reveals their impact in epidermolytic infections.
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