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Sperm characteristics and androgens in Acipenser ruthenus after induction of spermiation by carp pituitary extract or GnRHa implants

Alavi, Sayyed Mohammad Hadi
Author Alavi, Sayyed Mohammad Hadi South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic. alavi@frov.jcu.cz
Hatef, Azadeh
Author Hatef, Azadeh South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Mylonas, Constantinos C
Author Mylonas, Constantinos C Institute of Aquaculture, Hellenic Center for Marine Research, Thalassocosmos, P.O. Box 2214, 71003, Heraklion, Crete, Greece
Gela, David
Author Gela, David South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Papadaki, Maria
Author Papadaki, Maria Institute of Aquaculture, Hellenic Center for Marine Research, Thalassocosmos, P.O. Box 2214, 71003, Heraklion, Crete, Greece
Rodina, Marek
Author Rodina, Marek South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Kašpar, Vojtech
Author Kašpar, Vojtech South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Pšenička, Martin
Author Pšenička, Martin South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Podhorec, Peter
Author Podhorec, Peter South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic
Linhart, Otomar
Author Linhart, Otomar South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, 389 25, Vodnany, Czech Republic

Fish physiology and biochemistry. 2012 Dec ; 38 (6) : 1655-1666. [epub] 20120605

Fish Physiol Biochem
ISSN 1573-5168 | 0920-1742
Source

Language English Country Netherlands Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid22665160

PubMed 22665160
DOI 10.1007/s10695-012-9662-9
PII: 10.1007/s10695-012-9662-9
Knihovny.cz E-resources

MeSH
Semen Analysis veterinary MeSH
Gonadotropin-Releasing Hormone administration & dosage pharmacology MeSH
Drug Implants MeSH
Sperm Motility MeSH
Sexual Maturation drug effects MeSH
Fishes blood physiology MeSH
Spermatogenesis drug effects physiology MeSH
Spermatozoa drug effects physiology MeSH
Testosterone analogs & derivatives blood metabolism MeSH
Animals MeSH
Check Tag
Male MeSH
Animals MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
11-ketotestosterone MeSH Browser
Gonadotropin-Releasing Hormone MeSH
Drug Implants MeSH
Testosterone MeSH

Spermiation and changes in androgen (testosterone, T and 11-ketotestosterone, 11-KT) levels were studied in sterlet (Acipenser ruthenus) treated with GnRH agonist implants (DAla(6)-Pro(9)-LHRHa) at 25 and 75 μg kg(-1) b.w. and compared with those males treated with 4 mg kg(-1) b.w. of carp pituitary extract (CPE) and 3 pellets of Ovopel kg(-1) b.w., which contains DAla(6)-Pro(9)NEt-mGnRH and metoclopramide. Sperm quality (sperm mass, spermatozoa concentration and sperm motility and velocity) was evaluated 24, 48 and 72 h after hormonal treatments. Males did not release sperm in the control group injected with physiological solution, while sperm could not be collected 7 days after treatments in all hormonally treated groups. Spermiation rates were 100 % in the CPE and Ovopel groups and 25-50 % in the GnRHa-treated groups. Sperm production was significantly lower in the GnRHa-treated groups than in the CPE and Ovopel groups and decreased 72 h after hormonal treatment. Sperm motility and velocity were higher in the Ovopel and GnRHa (75 μg) groups compared to the CPE and GnRHa (25 μg) groups and decreased 72 h after hormonal treatment. Androgens were only affected in spermiating males and changed in the Ovopel and GnRHa (75 μg) after hormonal treatment. Significant correlations were observed between sperm production, sperm motility and sperm velocity, but not androgens. The present study suggests involvement of dopamine in sturgeon spawning. Additionally, better sperm quality observed in the Ovopel group and particularly sperm motility in the GnRHa (75 μg) suggests enhancement of sperm quality in sturgeon treated with GnRHa. Therefore, further study is needed to induce fully spermiation using GnRHa implants in combination with a dopamine inhibitor.

Institute of Aquaculture Hellenic Center for Marine Research Thalassocosmos P O Box 2214 71003 Heraklion Crete Greece

South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses Research Institute of Fish Culture and Hydrobiology Faculty of Fisheries and Protection of Waters University of South Bohemia 389 25 Vodnany Czech Republic

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