Preparation of stable Pd nanocubes and their use in biological labeling
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22771526
DOI
10.1016/j.colsurfb.2012.05.023
PII: S0927-7765(12)00311-6
Knihovny.cz E-resources
- MeSH
- Staining and Labeling methods MeSH
- Bromides chemistry MeSH
- HeLa Cells MeSH
- Immunoglobulins chemistry MeSH
- Immunoconjugates chemistry MeSH
- Nuclear Proteins analysis MeSH
- Colloids MeSH
- Metal Nanoparticles chemistry ultrastructure MeSH
- Ascorbic Acid chemistry MeSH
- Humans MeSH
- Microtomy MeSH
- Nucleophosmin MeSH
- Palladium chemistry MeSH
- Image Processing, Computer-Assisted MeSH
- Povidone chemistry MeSH
- Potassium Compounds chemistry MeSH
- Microscopy, Electron, Transmission MeSH
- Particle Size MeSH
- Water MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bromides MeSH
- Immunoglobulins MeSH
- Immunoconjugates MeSH
- Nuclear Proteins MeSH
- Colloids MeSH
- Ascorbic Acid MeSH
- NPM1 protein, human MeSH Browser
- Nucleophosmin MeSH
- Palladium MeSH
- potassium bromide MeSH Browser
- Povidone MeSH
- Potassium Compounds MeSH
- tetrachloropalladium MeSH Browser
- Water MeSH
Stable Pd nanocubes (PdNC) with the average size ~15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.
References provided by Crossref.org
Simultaneous detection of multiple targets for ultrastructural immunocytochemistry
