Preparation of stable Pd nanocubes and their use in biological labeling
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22771526
DOI
10.1016/j.colsurfb.2012.05.023
PII: S0927-7765(12)00311-6
Knihovny.cz E-zdroje
- MeSH
- barvení a značení metody MeSH
- bromidy chemie MeSH
- HeLa buňky MeSH
- imunoglobuliny chemie MeSH
- imunokonjugáty chemie MeSH
- jaderné proteiny analýza MeSH
- koloidy MeSH
- kovové nanočástice chemie ultrastruktura MeSH
- kyselina askorbová chemie MeSH
- lidé MeSH
- mikrotomie MeSH
- nukleofosmin MeSH
- palladium chemie MeSH
- počítačové zpracování obrazu MeSH
- povidon chemie MeSH
- sloučeniny draslíku chemie MeSH
- transmisní elektronová mikroskopie MeSH
- velikost částic MeSH
- voda MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bromidy MeSH
- imunoglobuliny MeSH
- imunokonjugáty MeSH
- jaderné proteiny MeSH
- koloidy MeSH
- kyselina askorbová MeSH
- NPM1 protein, human MeSH Prohlížeč
- nukleofosmin MeSH
- palladium MeSH
- potassium bromide MeSH Prohlížeč
- povidon MeSH
- sloučeniny draslíku MeSH
- tetrachloropalladium MeSH Prohlížeč
- voda MeSH
Stable Pd nanocubes (PdNC) with the average size ~15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.
Citace poskytuje Crossref.org
Simultaneous detection of multiple targets for ultrastructural immunocytochemistry
