Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22820294
DOI
10.1016/j.molbiopara.2012.07.002
PII: S0166-6851(12)00203-4
Knihovny.cz E-resources
- MeSH
- Alleles MeSH
- Species Specificity MeSH
- Genetic Variation MeSH
- Genotype MeSH
- Microsatellite Repeats genetics MeSH
- Mice MeSH
- Nematode Infections parasitology veterinary MeSH
- Rodent Diseases parasitology MeSH
- Oxyuroidea genetics MeSH
- Genetics, Population MeSH
- High-Throughput Nucleotide Sequencing methods MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Syphacia obvelata is a common gastro-intestinal parasitic nematode of the house mouse (Mus musculus), a prime model rodent species. Investigations of the genetic structure, variability of parasite populations and other biological aspects of this host-parasite system are limited due to the lack of genetic resources for S. obvelata. To fill this gap, we developed a set of microsatellite markers for S. obvelata, using a 454 pyrosequencing approach. We designed three multiplex panels allowing genotyping of 10 polymorphic loci and scrutinized them on 42 samples from two different regions inhabited by two different house mouse subspecies (Mus musculus musculus and M. m. domesticus). The numbers of alleles ranged from 2 to 6 with mean observed heterozygosities 0.1476 and 0.2095 for domesticus and musculus worms, respectively. The described markers will facilitate further studies on population biology and co-evolution of this host-parasite system.
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