A new F-actin structure in fungi: actin ring formation around the cell nucleus of Cryptococcus neoformans
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23155113
DOI
10.1093/jmicro/dfs074
PII: dfs074
Knihovny.cz E-resources
- MeSH
- Actins analysis MeSH
- Bridged Bicyclo Compounds, Heterocyclic pharmacology MeSH
- Cell Nucleus ultrastructure MeSH
- Cryptococcus neoformans drug effects physiology ultrastructure MeSH
- Dimethyl Sulfoxide pharmacology MeSH
- Microscopy, Electron MeSH
- Phalloidine analogs & derivatives MeSH
- Microscopy, Fluorescence MeSH
- Actin Cytoskeleton ultrastructure MeSH
- Microtubules drug effects MeSH
- Tubulin Modulators pharmacology MeSH
- Marine Toxins pharmacology MeSH
- Nocodazole pharmacology MeSH
- Rhodamines MeSH
- Free Radical Scavengers pharmacology MeSH
- Thiazolidines pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- Bridged Bicyclo Compounds, Heterocyclic MeSH
- Dimethyl Sulfoxide MeSH
- Phalloidine MeSH
- latrunculin A MeSH Browser
- Tubulin Modulators MeSH
- Marine Toxins MeSH
- Nocodazole MeSH
- rhodamine-phalloidin MeSH Browser
- Rhodamines MeSH
- Free Radical Scavengers MeSH
- Thiazolidines MeSH
The F-actin cytoskeleton of Cryptococcus neoformans is known to comprise actin cables, cortical patches and cytokinetic ring. Here, we describe a new F-actin structure in fungi, a perinuclear F-actin collar ring around the cell nucleus, by fluorescent microscopic imaging of rhodamine phalloidin-stained F-actin. Perinuclear F-actin rings form in Cryptococcus neoformans treated with the microtubule inhibitor Nocodazole or with the drug solvent dimethyl sulfoxide (DMSO) or grown in yeast extract peptone dextrose (YEPD) medium, but they are absent in cells treated with Latrunculin A. Perinuclear F-actin rings may function as 'funicular cabin' for the cell nucleus, and actin cables as intracellular 'funicular' suspending nucleus in the central position in the cell and moving nucleus along the polarity axis along actin cables.
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