The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- arginin imunologie farmakologie MeSH
- buněčné linie MeSH
- dieta MeSH
- endotoxiny imunologie MeSH
- extracelulárním signálem regulované MAP kinasy metabolismus MeSH
- fosfolipasy typu C metabolismus MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- oxid dusnatý metabolismus MeSH
- peritoneální makrofágy účinky léků imunologie MeSH
- peritonitida imunologie MeSH
- signální transdukce účinky léků imunologie MeSH
- synthasa oxidu dusnatého genetika metabolismus MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- arginin MeSH
- endotoxiny MeSH
- extracelulárním signálem regulované MAP kinasy MeSH
- fosfolipasy typu C MeSH
- oxid dusnatý MeSH
- synthasa oxidu dusnatého MeSH
It is known that cells and organisms can indirectly "sense" changes in L-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by L-arginine in mammalian cells have not yet been elucidated. We investigated the effect of L-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that L-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. L-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca(2+), as well as inducible nitric oxide synthase expression. Interestingly, the effect of L-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, L-arginine was shown to modulate the response of macrophages toward lipopolysaccharide via the activation of G-protein-coupled receptors. According to our data, we concluded that L-arginine availability plays a key role in the initiation of intracellular signaling pathways that trigger the lipopolysaccharide-induced inflammatory responses in murine macrophages. Although macrophages are partially stimulated in the absence of extracellular L-arginine, the presence of this amino acid significantly accelerates the sensitivity of macrophages to bacterial endotoxin.
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Clin Nutr. 2004 Aug;23(4):561-9 PubMed
Mol Cell. 2003 Mar;11(3):619-33 PubMed
Cell Signal. 2001 Feb;13(2):85-94 PubMed
Biochem Biophys Res Commun. 2000 Sep 7;275(3):715-9 PubMed
Int J Obes Relat Metab Disord. 2003 Dec;27 Suppl 3:S35-40 PubMed
Surgery. 1981 Aug;90(2):244-51 PubMed
Immunobiology. 2011 Apr;216(4):457-65 PubMed
Free Radic Biol Med. 2007 Apr 15;42(8):1290-302 PubMed
Respir Res. 2006 Jun 09;7:87 PubMed
J Physiol Pharmacol. 2009 Mar;60(1):143-50 PubMed
Eur J Immunol. 2006 Jun;36(6):1516-26 PubMed
Arterioscler Thromb Vasc Biol. 2004 Jan;24(1):23-8 PubMed
J Mol Med (Berl). 2007 Jul;85(7):723-32 PubMed
Ann N Y Acad Sci. 1999 Apr 30;868:667-76 PubMed
EMBO J. 2000 Dec 1;19(23):6622-33 PubMed
Br J Pharmacol. 2007 Mar;150(6):798-807 PubMed
Surgery. 1993 Aug;114(2):155-9; discussion 160 PubMed
Neuro Endocrinol Lett. 2008 Oct;29(5):790-3 PubMed
Nat Rev Immunol. 2005 Aug;5(8):641-54 PubMed
Clin Chem. 2009 Aug;55(8):1539-45 PubMed
Biochem Biophys Res Commun. 2004 Jan 9;313(2):447-52 PubMed
ScientificWorldJournal. 2011;11:2443-57 PubMed
Biochem J. 1998 Nov 15;336 ( Pt 1):1-17 PubMed
Immunobiology. 2007;212(9-10):795-812 PubMed
Nutrition. 2002 Jul-Aug;18(7-8):631-5 PubMed
J Immunol. 2003 Nov 1;171(9):4561-8 PubMed
J Immunol. 1996 Jun 1;156(11):4457-65 PubMed
J Nutr. 2004 Oct;134(10 Suppl):2842S-2847S; discussion 2853S PubMed
Immunobiology. 2009;214(9-10):822-7 PubMed
Traffic. 2006 Jun;7(6):686-98 PubMed
Ann N Y Acad Sci. 1998 Jun 30;851:139-46 PubMed
Biochem J. 2000 Oct 1;351(Pt 1):1-12 PubMed
J Nutr. 2007 Jun;137(6 Suppl 2):1602S-1609S PubMed
J Biomed Mater Res A. 2007 Feb;80(2):381-90 PubMed
Cell Signal. 2000 Jan;12(1):1-13 PubMed
Biochem J. 1992 Jan 1;281 ( Pt 1):45-8 PubMed
Eur J Pharmacol. 2004 Oct 1;500(1-3):255-66 PubMed
Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4843-8 PubMed
J Nutr. 2000 Jul;130(7):1827-30 PubMed
Biochem J. 2003 Jul 1;373(Pt 1):1-18 PubMed
Nature. 2002 Mar 14;416(6877):199-202 PubMed
Curr Opin Cell Biol. 1997 Apr;9(2):240-6 PubMed
Sci Signal. 2010 Aug 17;3(135):ra61 PubMed
Curr Opin Clin Nutr Metab Care. 2002 Jan;5(1):77-84 PubMed
Am J Physiol. 1995 Sep;269(3 Pt 1):C750-6 PubMed
Biochem Pharmacol. 2006 Nov 30;72(11):1493-505 PubMed
Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):9982-7 PubMed
J Biol Chem. 2001 May 11;276(19):15881-5 PubMed
Biochem J. 1989 Jul 1;261(1):293-6 PubMed
Pharm Res. 2002 Jun;19(6):825-31 PubMed
