AFM imaging and analysis of local mechanical properties for detection of surface pattern of functional groups
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23498219
DOI
10.1016/j.msec.2013.01.006
PII: S0928-4931(13)00019-2
Knihovny.cz E-resources
- MeSH
- Fibronectins chemistry MeSH
- Mechanical Phenomena * MeSH
- Microscopy, Atomic Force methods MeSH
- Nanospheres ultrastructure MeSH
- Surface Properties MeSH
- Streptavidin chemistry MeSH
- Gold MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fibronectins MeSH
- Streptavidin MeSH
- Gold MeSH
In this work we evaluate the applicability of different atomic force microscopy (AFM) modes, such as Phase Shift Imaging, Atomic Force Acoustic Microscopy (AFAM) and Force Spectroscopy, for mapping of the distribution pattern of low-molecular-weight biomimetic groups on polymer biomaterial surfaces. Patterns with either random or clustered spatial distribution of bioactive peptide group derived from fibronectin were prepared by surface deposition of functional block copolymer nano-colloids and grafted with RGDS peptide containing the sequence of amino acids arginine-glycine-aspartic acid-serine (conventionally labeled as RGDS) and carrying biotin as a tag. The biotin-tagged peptides were labeled with 40nm streptavidin-modified Au nanospheres. The peptide molecules were localized through the detection of bound Au nanospheres by AFM, and thus, the surface distribution of peptides was revealed. AFM techniques capable of monitoring local mechanical properties of the surface were proved to be the most efficient for identification of Au nano-markers. The efficiency was successfully demonstrated on two different patterns, i.e. random and clustered distribution of RGDS peptides on structured surface of the polymer biomaterial.
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