Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue
Jazyk angličtina Země Řecko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23620014
DOI
10.3892/ijmm.2013.1363
Knihovny.cz E-zdroje
- MeSH
- adenokarcinom plic MeSH
- adenokarcinom genetika MeSH
- dexamethason farmakologie MeSH
- inzulin farmakologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nádory plic genetika MeSH
- plíce účinky léků metabolismus MeSH
- protein A asociovaný s plicním surfaktantem genetika metabolismus MeSH
- protein B asociovaný s plicním surfaktantem genetika metabolismus MeSH
- protein C asociovaný s plicním surfaktantem genetika metabolismus MeSH
- protein D asociovaný s plicním surfaktantem genetika metabolismus MeSH
- proteiny asociované s plicním surfaktantem genetika metabolismus MeSH
- regulace genové exprese u nádorů účinky léků MeSH
- regulace genové exprese účinky léků MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dexamethason MeSH
- inzulin MeSH
- protein A asociovaný s plicním surfaktantem MeSH
- protein B asociovaný s plicním surfaktantem MeSH
- protein C asociovaný s plicním surfaktantem MeSH
- protein D asociovaný s plicním surfaktantem MeSH
- proteiny asociované s plicním surfaktantem MeSH
In this study, the effects of insulin and dexamethasone on the expression and mRNA transcription of 4 pulmonary surfactant-associated proteins [surfactant protein (SFTP)A, SFTPB, SFTPC and SFTPD] were examined. The commercially available cell lines, A549 and H441, were used as acceptable models of lung surfactant-producing cells. Subsequently, the effects of insulin on the expression of surfactant-associated proteins were examined in patients with lung adenocarcinoma during lung resection. Our results demonstrated the inhibitory effects of insulin on the transcription of the SFTPB, SFTPC and SFTPD genes in H441 cells and the SFTPB gene in A549 cells. Treatment with insulin significantly decreased the protein expression of SFTPA1 and SFTPA2 in the H441 cells and that of proSFTPB in the A549 cells. Dexamethasone promoted the transcription of the SFTPB, SFTPC and SFTPD genes in the A549 and H441 cells and reduced the transcription of the SFTPA1 and SFTPA2 genes in the H441 cells (SFTPA mRNA expression was not detected in A549 cells). Furthermore, we demonstrated that the mRNA levels of the selected genes were significantly lower in the cell lines compared to the lung tissue. A549 and H441 cells represent similar cell types. Yet, in our experiments, these cells reacted differently to insulin and/or dexamethasone treatment, and the mRNA levels of their main protein products, surfactant-associated proteins, were significantly lower than those in real tissue. Therefore, the results obtained in this study challenge the suitability of A549 and H441 cells as models of type II pneumocytes and Clara cells, respectively. However, we successfully demonstrate the possibility of studying the effects of insulin on pulmonary surfactant-associated genes and proteins in patients with lung adenocarcinoma.
Citace poskytuje Crossref.org
