The growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Culture Techniques MeSH
- Antigens, CD metabolism MeSH
- Adult Stem Cells metabolism transplantation MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Limbus Corneae cytology MeSH
- Mesenchymal Stem Cells physiology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Nanofibers chemistry MeSH
- Nylons chemistry MeSH
- Cell Separation MeSH
- Tissue Scaffolds chemistry MeSH
- Mesenchymal Stem Cell Transplantation * MeSH
- Cell Shape MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, CD MeSH
- Nylons MeSH
The injured or otherwise damaged cornea is healed by limbal stem cells (LSC). If the limbus where LSC reside is also damaged or nonfunctional, the cornea cannot heal properly and this defect leads to impaired vision that can result in blindness. The only way to treat total LSC deficiency is by transplantation of limbal tissue or a transfer of LSC. Recently, mesenchymal stem cells (MSC) have been shown as another promising source of stem cells for corneal healing and regeneration. Here, we describe a protocol for the use of polyamide 6/12 nanofiber scaffolds for the growth of MSC and LSC, and for their transfer onto a mechanically damaged ocular surface in the experimental mouse model.
References provided by Crossref.org
Mesenchymal stem cells, nanofiber scaffolds and ocular surface reconstruction
