Endocrine regulation of non-circadian behavior of circadian genes in insect gut
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23811190
DOI
10.1016/j.jinsphys.2013.06.004
PII: S0022-1910(13)00136-4
Knihovny.cz E-resources
- Keywords
- Corpus allatum, Cryptochrome, Diapause, Endocrine glands, Par domain protein 1, Pars intercerebralis, Photoperiod,
- MeSH
- Circadian Rhythm MeSH
- Corpora Allata physiology MeSH
- Gastrointestinal Tract metabolism MeSH
- Heteroptera metabolism MeSH
- Genes, Insect MeSH
- Insect Proteins genetics metabolism MeSH
- Cryptochromes genetics metabolism MeSH
- Ovary physiology MeSH
- Gene Expression Regulation * MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Insect Proteins MeSH
- Cryptochromes MeSH
The linden bug Pyrrhocoris apterus exhibits a robust diapause response to photoperiod. Photoperiod strongly affected basal levels of circadian gene transcripts in the gut, via the neuroendocrine system. Cryptochrome 2 (cry2) mRNA level was much higher in diapause promoting short days (SD) than in reproduction promoting long days (LD), while Par Domain Protein 1 (Pdp1) mRNA level was higher in LD than in SD. The effect of photoperiod on gene expression was mediated by the neurosecretory cells of the pars intercerebralis (PI) and the juvenile hormone (JH) producing corpus allatum (CA). In LD-females, CA ablation resulted in SD-like levels of gene transcripts, while PI ablation had little effect. Conversely, in SD-females, CA ablation had only a little effect, while PI ablation resulted in LD-like levels of gene transcripts. Thus, the CA is responsible for LD-like characteristics of gene expression in reproducing females and the PI is responsible for SD-like characteristics of gene expression in diapausing females. A simultaneous ablation of both PI and CA revealed two roles of PI in SD-females: (1) inhibition of CA, and (2) weak CA-independent stimulation of cry2 mRNA. Overall, our results indicate that peripheral circadian gene expression in the gut reflects the physiological state of females (with respect to diapause or reproduction) rather than the external light-dark cycle.
J Insect Physiol. 2013 Oct;59(10):1087 PubMed
References provided by Crossref.org
Light and Temperature Synchronizes Locomotor Activity in the Linden Bug, Pyrrhocoris apterus
