Parameters characterizing the structure of the decomposer food web, biomass of the soil microflora (bacteria and fungi) and soil micro-, meso- and macrofauna were studied at 14 non-reclaimed 1- 41-year-old post-mining sites near the town of Sokolov (Czech Republic). These observations on the decomposer food webs were compared with knowledge of vegetation and soil microstructure development from previous studies. The amount of carbon entering the food web increased with succession age in a similar way as the total amount of C in food web biomass and the number of functional groups in the food web. Connectance did not show any significant changes with succession age, however. In early stages of the succession, the bacterial channel dominated the food web. Later on, in shrub-dominated stands, the fungal channel took over. Even later, in the forest stage, the bacterial channel prevailed again. The best predictor of fungal bacterial ratio is thickness of fermentation layer. We argue that these changes correspond with changes in topsoil microstructure driven by a combination of plant organic matter input and engineering effects of earthworms. In early stages, soil is alkaline, and a discontinuous litter layer on the soil surface promotes bacterial biomass growth, so the bacterial food web channel can dominate. Litter accumulation on the soil surface supports the development of the fungal channel. In older stages, earthworms arrive, mix litter into the mineral soil and form an organo-mineral topsoil, which is beneficial for bacteria and enhances the bacterial food web channel.

Institute of Soil Biology Biology Centre Academy of Sciences of the Czech Cepublic České Budějovice Czech Republic ; Institute for Environmental Studies Charles University Prague Czech Republic

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Clements EF (1936) Nature and structure of the climax. J Ecol 24: 552–584.

Glenn-Lewin DC, Peet RK, Veblen TT (1992) Plant succession, theory and prediction. London: Chapman and Hall. 352 p.

Luken JO (1990) Directing ecological succession. London: Chapman and Hall. 251 p.

Odum EP (1969) The strategy of ecosystem development. Science 164: 262–270. PubMed

Neutel AM, Heesterbeek JAP, van de Koppel J, Hoenderboom G, Vos A, et al. (2007) Reconciling complexity with stability in naturally assembling food webs. Nature 449: 599–603. PubMed

Chapman SK, Langley JA, Hart SC, Koch GW (2006) Plants actively control nitrogen cycling: uncorking the microbial bottleneck. New Phytol 169: 27–34. PubMed

Ponge JF (2003) Humus form in terrestrial ecosystem: a framework to biodiversity. Soil Biol Biochem 35: 935–945.

De Deyn GB, Raaijmakers CE, Zoomer HR, Berg MP, de Ruiter PC, et al. (2003) Soil invertebrate fauna enhance grassland succession and diversity. Nature 422: 711–713. PubMed

Frouz J, Prach K, Pižl V, Háněl L, Starý J, et al. (2008) Interactions between soil development, vegetation and soil fauna during spontaneous succession in post mining sites. Eur J Soil Biol 44: 109–121.

Lavelle P, Bignell D, Lepage M, Wolters V, Roger P, et al. (1997) Soil function in a changing world: the role of invertebrate ecosystem engineers. Eur J Soil Biol 33: 159–193.

Wardle DA, Yeates GW (1993) The dual importance of competition and predation as regulatory forces in terrestrial ecosystems: evidence from decomposer food-webs. Oecologia 93: 303–306. PubMed

Moore JC, de Ruiter PC (2000) Invertebrates in detrital food webs along gradients of productivity. In: Coleman DC, Hendrix PF, editors. Invertebrates as webmasters in ecosystems. CABI Wallingford, UK. pp. 161–184.

Hedlund K, Griffiths B, Christensen S, Scheu S, Setälä H, et al. (2004) Trophic interactions in changing landscapes: responses of soil food webs. Basic Appl Ecol 5: 495–503.

Wardle DA, Yeates GW, Watson RN, Nicholson KS (1995) Development of the decomposer food-web, trophic relationships, and ecosystem properties during a three-year primary succession in sawdust. Oikos 73: 155–166.

Scheu S, Schaefer M (1998) Bottom-up control of the soil macrofauna community in a beechwood on limestone: manipulation of food resources. Ecology 79: 1573–1585.

Hobbie SE (1992) Effects of plant species on nutrient cycling. Trends Ecol Evol 7: 336–339. PubMed

Frouz J, Pižl V, Tajovský K (2007) The effect of earthworms and other saprophagous macrofauna on soil microstructure in reclaimed and un-reclaimed post-mining sites in Central Europe. Eur J Soil Biol 43: 184–189.

Frouz J, Elhottová D, Pižl V, Tajovský K, Šourková M, et al. (2007) The effect of liter quality and soil fauna composition on organic matter dynamic in post mining soil: a laboratory study. Appl Soil Ecol 37: 72–80.

Frouz J, Nováková A (2005) Development of soil microbial properties in topsoil layer during spontaneous succession in heaps after brown coal mining in relation to soil microstructure development. Geoderma 129: 54–64.

Vance ED, Brookes PC, Jenkinson DS (1987) An extraction method for measuring soil microbial biomass C. Soil Biol Biochem 19: 703–707.

Baldrian P, Trögl J, Frouz J, Šnajdr J, Valášková V, et al. (2008) Enzyme activities and microbial biomass in topsoil layer during spontaneous succession in spoil heaps after brown coal mining. Soil Biol Biochem 40: 2107–2115.

Adl SM, Acosta-Mercado D, Lynn DH (2007) Protozoa. In: Carter MR, Gregorich EG, editors. Soil sampling and methods of analysis. CRC Press. pp. 455–469.

Háněl L (1995) Secondary successional stages of soil nematodes in cambisols of South Bohemia. Nematologica 41: 197–218.

Yeates CW (1998) Feeding in free-living soil nematodes: a functional approach. In: Perry RN, Wricht DJ, editors. The physiology and biochemistry of free-living and parasitic nematodes. CABI publishing, Wallingford. pp. 245–269.

Háněl L (2003) Soil nematodes in cambisoil agroecosystem of the Czech Republic. Biologia 52: 205–216.

Frouz J (1999) Use of soil dwelling Diptera (Insecta, Diptera) as bioindicators: a review of ecological requirements and response to disturbance. Agr Ecosyst Environ 74: 167–186.

Makulec G (2002) The role of Lumbricus rubellus Hoffm. in determining biotic and abiotic properties of peat soils. Pol J Ecol 50: 301–339.

Pižl V (1992) Succession of earthworm population in abandoned fields. Soil Biol Biochem 24: 1623–1628.

Hunt HW, Coleman DC, Ingham ER, Ingham RE, Elliott ET, et al. (1987) The detrital food web in a shortgrass prairie. Biol Fertil Soils 3: 57–68.

Williams RJ, Martinez ND (2004) Stabilization of chaotic and non-permanent food-web dynamics. Eur Physical J B 38: 297–303.

Holtkamp R, Kardol P, van der Wal A, Dekker SC, van der Putten WH (2008) Soil food web structure during ecosystem development after land abandonment. Appl Soil Ecol 39: 23–34.

de Ruiter PC, Neutel AM, Moore JC (1995) Modelling food webs and nutrient cycling in agro-ecosystems. Trends Ecol Evol 9: 378–383. PubMed

Neutel AM, Heesterbeek JAP, de Ruiter PC (2002) Stability in real food webs: weak links in long loops. Science 296: 1120–1123. PubMed

May RM (1973) Qualitative stability in model ecosystems. Ecology 54: 638–641.

Bohlen PJ, Groffman PM, Fahey TJ, Fisk MC (2004) Ecosystem consequences of exotic earthworm invasion of north temperate forests. Ecosystems 7: 1–12.

Dempsey MA, Fisk MC, Fahey TJ (2011) Earthworms increase the ratio of bacteria to fungi in northern hardwood forest soils, primarily by eliminating the organic horizon. Soil Biol Biochem 43: 2135–2141.

Dunger W (1968) Die Entwicklung der Bodenfauna auf rekultivieren Kippen und Halden des Braunkohlentegebaues. Abh Ber Naturkundesmuseums Görlitz 43: 1–256.

Dunger W (1991) Zur Primärsukzession humiphager Tiergruppen auf Bergbauflächen. Zool Jahrb Syst 118: 423–447.

Frouz J, Keplin B, Pižl V, Tajovský K, Starý J, et al. (2001) Soil biota and upper soil layers development in two contrasting post-mining chronosequences. Ecol Eng 17: 275–284.

Rusek J (1971) Pedozootische Sukzessionen warend der Entwicklung von Ökosystemen. Pedobiologia 18: 426–433.

Aira M, McNamara N, Trevor P, Domínguez J (2009) Microbial communities of Lumbricus terrestris L. middens: structure, activity, and changes through time in relation to earthworm presence. J Soils Sediments 9: 54–61.

Prach K, Pyšek P, Šmilauer P (1999) Prediction of vegetation succession in human-disturbed habitats using an expert system. Restor Ecol 7: 15–23.

Prach K, Bartha S, Joyce CB, Pyšek P, van Diggelen R, et al. (2001) The role of spontaneous vegetation succession in ecosystem restoration: a perspective. Appl Veg Sci 4: 111–114.

Prach K, Pyšek P, Bastl M (2001) Spontaneous vegetation succession in human-disturbed habitats: a pattern across seres. Appl Veg Sci 4: 83–88.

Adl SM (2008) Setting the tempo in land remediation: short term and long term pattern in biodiversity recovery. Microbes Environ 23: 13–19. PubMed

Kaufmann R (2001) Invertebrate succession on an alpine glacier foreland. Ecology 82: 2261–2278.

Brussaard L (1998) Soil fauna, guilds, functional groups and ecosystem processes. Appl Soil Ecol 9: 123–135.

Worm B, Duffy JE (2003) Biodiversity, productivity and stability in real food webs. Trends Ecol Evol 18: 628–632.

Griffiths BS, Bonkowski M, Dobson G, Caul S (1999) Changes in soil microbial community structure in the presence of microbial-feeding nematodes and protozoa. Pedobiologia 25: 383–401.

Paustian K, Andrén O, Clarholm M, Hansson AC, Johansson G (1990) Carbon and nitrogen budgets of four agroecosystems with annual and perennial crops, with and without nitrogen fertilization. J Appl Ecol 27: 60–84.

Sohlenius B (1990) Influence of cropping system and nitrogen input on soil fauna and microorganisms in a Swedish arable soil. Biol Fertil Soils 9: 168–173.

Rooney N, McCann K, Gellner G, Moore JC (2006) Structural asymmetry and the stability of diverse food webs. Nature 442: 265–269. PubMed

Global distribution of soil fauna functional groups and their estimated litter consumption across biomes