Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm
Language English Country Canada Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
24313452
DOI
10.1139/cjm-2013-0635
Knihovny.cz E-resources
- MeSH
- Azides chemistry MeSH
- Biofilms * MeSH
- Campylobacter genetics isolation & purification physiology MeSH
- Intercalating Agents chemistry MeSH
- Microbial Viability * MeSH
- Multiplex Polymerase Chain Reaction methods MeSH
- Propidium analogs & derivatives chemistry MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 8-azidoethidium MeSH Browser
- Azides MeSH
- Intercalating Agents MeSH
- propidium monoazide MeSH Browser
- Propidium MeSH
This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA-PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells.
References provided by Crossref.org
