Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification
Language English Country Great Britain, England Media print-electronic
Document type Evaluation Study, Journal Article
PubMed
24365693
DOI
10.1016/j.jflm.2013.10.016
PII: S1752-928X(13)00290-4
Knihovny.cz E-resources
- Keywords
- Bacteria, Forensic, Interlaboratory evaluation, Multiplex PCR, Vaginal fluids, mfDNA,
- MeSH
- Cervix Mucus microbiology MeSH
- DNA, Bacterial genetics MeSH
- Adult MeSH
- Enterococcus faecalis genetics isolation & purification MeSH
- Real-Time Polymerase Chain Reaction * MeSH
- Laboratories standards MeSH
- Lactobacillus genetics isolation & purification MeSH
- Humans MeSH
- Multiplex Polymerase Chain Reaction * MeSH
- Forensic Medicine standards MeSH
- Staphylococcus genetics isolation & purification MeSH
- Streptococcus genetics isolation & purification MeSH
- Vagina microbiology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- DNA, Bacterial MeSH
Identification of vaginal fluids is an important step in the process of sexual assaults confirmation. Advances in both microbiology and molecular biology defined technical approaches allowing the discrimination of body fluids. These protocols are based on the identification of specific bacterial communities by microfloraDNA (mfDNA) amplification. A multiplex real time-PCR assay (ForFLUID kit) has been developed for identifying biological fluids and for discrimination among vaginal, oral and fecal samples. In order to test its efficacy and reliability of the assay in the identification of vaginal fluids, an interlaboratory evaluation has been performed on homogeneous vaginal swabs. All the involved laboratories were able to correctly recognize all the vaginal swabs, and no false positives were identified when the assay was applied on non-vaginal samples. The assay represents an useful molecular tool that can be easily adopted by forensic geneticists involved in vaginal fluid identification.
Carabinieri Reparto Investigazioni Scientifiche di Roma 5 le di Tor di Quinto 119 00191 Roma Italy
Department of Forensic Medicine Yonsei University College of Medicine Seoul 120 752 South Korea
Molecular Digital Diagnostics s r l Via de Lellis 01100 Viterbo Italy
National Institute of Criminalistics and Criminology Vilvoordsesteenweg 100 1120 Brussel Belgium
Universität Zürich Institut für Rechtsmedizin Winterthurerstrasse 190 52 CH 8057 Zürich Switzerland
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