Phosphoenolpyruvate carboxylase, NADP-malic enzyme, and pyruvate, phosphate dikinase are involved in the acclimation of Nicotiana tabacum L. to drought stress
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24484954
DOI
10.1016/j.jplph.2013.10.017
PII: S0176-1617(13)00440-9
Knihovny.cz E-zdroje
- Klíčová slova
- Drought, NADP-malic enzyme, Nicotiana tabacum L., Phosphoenolpyruvate carboxylase, Pyruvate, phosphate dikinase,
- MeSH
- aklimatizace MeSH
- chloroplasty metabolismus MeSH
- fosfoenolpyruvátkarboxylasa genetika metabolismus MeSH
- fyziologický stres fyziologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- listy rostlin enzymologie MeSH
- malátdehydrogenasa genetika metabolismus MeSH
- období sucha * MeSH
- pyruvátfosfátdikinasa genetika metabolismus MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné proteiny genetika metabolismus MeSH
- tabák enzymologie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfoenolpyruvátkarboxylasa MeSH
- malátdehydrogenasa MeSH
- malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) MeSH Prohlížeč
- pyruvátfosfátdikinasa MeSH
- rostlinné proteiny MeSH
Drought stress is one of the most frequent forms of abiotic stresses, which occurs under condition of limited water availability. In this work, the possible participation of phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC), NADP-malic enzyme (EC 1.1.1.40; NADP-ME), and pyruvate, phosphate dikinase (EC 2.7.9.1; PPDK) in response to drought of tobacco plants (Nicotiana tabacum L., cv. W38) was investigated. Enzyme specific activities in tobacco leaves of drought stressed plants were significantly increased after 11 days of stress, PEPC 2.3-fold, NADP-ME 3.9-fold, and PPDK 2.7-fold compared to control plants. The regulation of PEPC and NADP-ME activities were studied on transcriptional level by the quantitative RT PCR and on translational level - immunochemically. The amount of NADP-ME protein and transcription of mRNA for chloroplastic NADP-ME isoform were increased indicating their enhanced synthesis de novo. On the other hand, mRNA for cytosolic isoform of NADP-ME was decreased. The changes in PEPC protein and PEPC mRNA were not substantial. Therefore regulation of PEPC activity by phosphorylation was evaluated and found to be involved in the stress response. During recovery, activities of the tested enzymes returned close to their basal levels.
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