Nitrolancea hollandica gen. nov., sp. nov., a chemolithoautotrophic nitrite-oxidizing bacterium isolated from a bioreactor belonging to the phylum Chloroflexi
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bioreactors microbiology MeSH
- Chemoautotrophic Growth MeSH
- Chloroflexi classification genetics isolation & purification ultrastructure MeSH
- DNA, Bacterial genetics MeSH
- Nitrites metabolism MeSH
- Phylogeny * MeSH
- Fatty Acids chemistry MeSH
- Molecular Sequence Data MeSH
- Nitrification MeSH
- Oxidation-Reduction MeSH
- Peptidoglycan chemistry MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Vitamin K 2 analogs & derivatives chemistry MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Netherlands MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Nitrites MeSH
- Fatty Acids MeSH
- Peptidoglycan MeSH
- RNA, Ribosomal, 16S MeSH
- Vitamin K 2 MeSH
- vitamin MK 8 MeSH Browser
A novel nitrite-oxidizing bacterium (NOB), strain Lb(T), was isolated from a nitrifying bioreactor with a high loading of ammonium bicarbonate in a mineral medium with nitrite as the energy source. The cells were oval (lancet-shaped) rods with pointed edges, non-motile, Gram-positive (by staining and from the cell wall structure) and non-spore-forming. Strain Lb(T) was an obligately aerobic, chemolitoautotrophic NOB, utilizing nitrite or formate as the energy source and CO2 as the carbon source. Ammonium served as the only source of assimilated nitrogen. Growth with nitrite was optimal at pH 6.8-7.5 and at 40 °C (maximum 46 °C). The membrane lipids consisted of C20 alkyl 1,2-diols with the dominant fatty acids being 10MeC18 and C(18 : 1)ω9. The peptidoglycan lacked meso-DAP but contained ornithine and lysine. The dominant lipoquinone was MK-8. Phylogenetic analyses of the 16s rRNA gene sequence placed strain Lb(T) into the class Thermomicrobia of the phylum Chloroflexi with Sphaerobacter thermophilus as the closest relative. On the basis of physiological and phylogenetic data, it is proposed that strain Lb(T) represents a novel species of a new genus, with the suggested name Nitrolancea hollandica gen. nov., sp. nov. The type strain of the type species is Lb(T) ( = DSM 23161(T) = UNIQEM U798(T)).
Department of Biotechnology TU Delft The Netherlands
Department of Microbial Ecology Ecology Centre University of Vienna Vienna Austria
Department of Water Technology ICT Prague Czech Republic
Microbiology Department Faculty of Biology Lomonosov Moscow State University Moscow Russia
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