Microsporum aenigmaticum sp. nov. from M. gypseum complex, isolated as a cause of tinea corporis
Language English Country Great Britain, England Media print-electronic
Document type Case Reports, Journal Article, Research Support, Non-U.S. Gov't
PubMed
24625678
DOI
10.1093/mmy/myt033
PII: myt033
Knihovny.cz E-resources
- Keywords
- Arthroderma, Onygenales, geophilic dermatophytes, keratinophilic fungi, skin infection, sterile fungi,
- MeSH
- Phylogeny MeSH
- Middle Aged MeSH
- Humans MeSH
- DNA, Ribosomal Spacer chemistry genetics MeSH
- Microsporum classification genetics isolation & purification MeSH
- Molecular Sequence Data MeSH
- Mycological Typing Techniques MeSH
- DNA, Ribosomal chemistry genetics MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Tinea diagnosis microbiology MeSH
- Tubulin genetics MeSH
- Wrist microbiology pathology MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Case Reports MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Ribosomal Spacer MeSH
- DNA, Ribosomal MeSH
- Tubulin MeSH
An undescribed Microsporum species was isolated from skin scales recovered from a 40-mm large, annular, scaling lesion on the wrist of a 46-year-old woman. The risk factors for dermatophyte infection in the patient were frequent work in the garden, hunting, and contact with dogs and horses. Direct microscopic examination of the scales revealed the presence of dermatophyte hyphae; when the samples were cultured, a morphologically similar fungus grew on all slants in pure culture. Both of these findings strongly suggested that the isolate was the true causal agent of infection. The possible geophilic nature of the species was based on phylogenetic analysis (internal transcribed spacer region of rDNA and β-tubulin gene) that placed it in between species of the M. gypseum complex. However, its divergencies from all other Microsporum species exceeded 4% base pairs. Based on β-tubulin phylogeny, the isolated species is a sister to M. gypseum. The species produces abundant chlamydospores and clumps of hyphae similar to those of ascomatal primordia but no conidia and ascospores. The species was unable to grow at 37°C and does not grow on T6 basal medium, which is unlike other Microsporum species; hair perforation and urease tests were positive. The addition of histidine to the T6 medium resulted in rapid growth of the fungus. The phylogenetic evidence, morphology, growth parameters, and physiology justified the proposal that the isolate is a new species, M. aenigmaticum, sp. nov.
References provided by Crossref.org
GENBANK
HG518404, HG518405, HG518406, HG518407, HG518408, HG518409, HG518410, HG518413, HG518414, HG518415, HG518416, HG518417, HG518418, HG518419, HG518420, HG518421, HG518422, HG518423, HG518424, HG793055, HG793056, HG793057, HG793058, HG793059, HG793060
