DNA barcoding of five common stored-product pest species of genus Cryptolestes (Coleoptera: Laemophloeidae)
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24725375
DOI
10.1017/s0007485314000224
PII: S0007485314000224
Knihovny.cz E-zdroje
- MeSH
- brouci genetika MeSH
- fylogeneze MeSH
- genetická variace MeSH
- haplotypy MeSH
- klasifikace metody MeSH
- mitochondriální DNA chemie MeSH
- sekvenční analýza DNA MeSH
- sekvenční seřazení MeSH
- taxonomické DNA čárové kódování MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mitochondriální DNA MeSH
Several species of the genus Cryptolestes Ganglbauer, 1899 (Coleoptera: Laemophloeidae) are commonly found in stored products. In this study, five species of Cryptolestes, with almost worldwide distribution, were obtained from laboratories in China, Czech Republic and the USA: Cryptolestes ferrugineus (Stephens, 1831), Cryptolestes pusillus (Schönherr, 1817), Cryptolestes turcicus (Grouvelle, 1876), Cryptolestes pusilloides (Steel & Howe, 1952) and Cryptolestes capensis (Waltl, 1834). Molecular identification based on a 658 bp fragment from the mitochondrial DNA cytochrome c oxidase subunit I (COI) was adopted to overcome some problems of morphological identification of Cryptolestes species. The utility of COI sequences as DNA barcodes in discriminating the five Cryptolestes species was evaluated on adults and larvae by analysing Kimura 2-parameter distances, phylogenetic tree and haplotype networks. The results showed that molecular approaches based on DNA barcodes were able to accurately identify these species. This is the first study using DNA barcoding to identify Cryptolestes species and the gathered DNA sequences will complement the biological barcode database.
Academy of State Administration of Grain No 11 Baiwanzhuang Street Beijing China
Department of Entomology and Plant Pathology Oklahoma State University Stillwater OK USA
Department of Zoology Faculty of Science Charles University Prague Viničná 7 Prague Czech Republic
Institute of Animal and Plant Quarantine Chinese Academy of Inspection and Quarantine Beijing China
Bull Entomol Res. 2014 Oct;104(5):679 PubMed
Citace poskytuje Crossref.org