Real-time luminescence microspectroscopy monitoring of singlet oxygen in individual cells
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24954013
DOI
10.1039/c4pp00121d
Knihovny.cz E-resources
- MeSH
- Single-Cell Analysis instrumentation methods MeSH
- 3T3 Cells MeSH
- Fibroblasts metabolism MeSH
- Photochemical Processes MeSH
- Photosensitizing Agents MeSH
- Luminescence MeSH
- Microspectrophotometry instrumentation methods MeSH
- Mice MeSH
- Computer Systems MeSH
- Porphyrins MeSH
- Singlet Oxygen metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosensitizing Agents MeSH
- Porphyrins MeSH
- Singlet Oxygen MeSH
- tetra(4-N-methylpyridyl)porphine MeSH Browser
A new setup for direct microspectroscopic monitoring of singlet oxygen ((1)O2) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect (1)O2. The (1)O2 phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of (1)O2 phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence.
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