A new setup for direct microspectroscopic monitoring of singlet oxygen ((1)O2) has been developed in our laboratory using a novel near-infrared sensitive InGaAs 2D-array detector. An imaging spectrograph has been inserted in front of the 2D-array detector, which allows us to acquire spectral images where one dimension is spatial and the other is spectral. The work presents a detailed examination of sensitivity and noise characteristics of the setup and its ability to detect (1)O2. The (1)O2 phosphorescence-based images and near-infrared luminescence spectral images recorded from single TMPyP-containing fibroblast cells reflecting spectral changes during irradiation are demonstrated. The introduction of spectral images addresses the issue of a potential spectral overlap of (1)O2 phosphorescence with near-infrared-extended luminescence of the photosensitizer and provides a powerful tool for distinguishing and separating them, which can be applied to any photosensitizer manifesting near-infrared luminescence.
- MeSH
- Single-Cell Analysis instrumentation methods MeSH
- 3T3 Cells MeSH
- Fibroblasts metabolism MeSH
- Photochemical Processes MeSH
- Photosensitizing Agents MeSH
- Luminescence MeSH
- Microspectrophotometry instrumentation methods MeSH
- Mice MeSH
- Computer Systems MeSH
- Porphyrins MeSH
- Singlet Oxygen metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- MeSH
- Bacterial Infections and Mycoses diagnosis blood microbiology MeSH
- Time MeSH
- Microbiological Techniques methods instrumentation trends MeSH
- Microspectrophotometry methods instrumentation MeSH
- Signal Processing, Computer-Assisted MeSH
- Spectroscopy, Fourier Transform Infrared methods instrumentation MeSH
- MeSH
- Diagnostic Techniques, Surgical methods instrumentation statistics & numerical data MeSH
- Endoscopy MeSH
- Humans MeSH
- Microspectrophotometry methods instrumentation statistics & numerical data MeSH
- Minimally Invasive Surgical Procedures MeSH
- Multivariate Analysis MeSH
- Brain Neoplasms diagnosis surgery MeSH
- Check Tag
- Humans MeSH
- MeSH
- Cranial Nerves pathology MeSH
- Cricetinae MeSH
- Mathematics MeSH
- Microspectrophotometry methods statistics & numerical data MeSH
- Nerve Tissue pathology MeSH
- Scrapie pathology MeSH
- Spectroscopy, Fourier Transform Infrared methods statistics & numerical data MeSH
- Frozen Sections MeSH
- Animals MeSH
- Check Tag
- Cricetinae MeSH
- Animals MeSH
- MeSH
- alpha-Glucosidases diagnostic use MeSH
- Azo Compounds diagnostic use MeSH
- Electrophoresis, Polyacrylamide Gel * methods utilization MeSH
- Microscopy, Electron methods trends utilization MeSH
- Glycoside Hydrolases diagnostic use MeSH
- Histocytochemistry * methods trends utilization MeSH
- Catecholamines diagnostic use MeSH
- Clinical Enzyme Tests * methods trends utilization MeSH
- Microspectrophotometry methods trends utilization MeSH
- Cell Membrane Permeability MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Review MeSH
Acta oto-laryngologica, ISSN 0365-5237 suppl. 276, 1971
110 s. : il., tab., grafy ; 26 cm
- MeSH
- Bronchi anatomy & histology MeSH
- Epithelium physiology MeSH
- Microspectrophotometry utilization MeSH
- Conspectus
- Patologie. Klinická medicína
- NML Fields
- otorinolaryngologie
