Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25089958
DOI
10.1016/j.jchromb.2014.07.023
PII: S1570-0232(14)00480-2
Knihovny.cz E-resources
- Keywords
- Aqueous samples, GC–MS/MS analysis, Metabolites, Protozoa, Trimethylsilylation,
- MeSH
- Alanine analysis chemistry metabolism MeSH
- Glucose analysis chemistry metabolism MeSH
- Lactic Acid analysis chemistry metabolism MeSH
- Limit of Detection MeSH
- Organosilicon Compounds chemistry MeSH
- Gas Chromatography-Mass Spectrometry methods MeSH
- Reproducibility of Results MeSH
- Trimethylsilyl Compounds chemistry MeSH
- Trypanosoma brucei brucei chemistry metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alanine MeSH
- Glucose MeSH
- hexamethylsilazane MeSH Browser
- Lactic Acid MeSH
- N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide MeSH Browser
- Organosilicon Compounds MeSH
- Trimethylsilyl Compounds MeSH
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mg L(-1) for glucose to 0.85 mg L(-1) for lactate.
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